New compound from the plant origin of propolis from Lombok, Indonesia and its antibacterial activity

Sayaka Mizuno, Ryo Miyata, Kazuma Mukaide, Sari Honda, Agus Sukito, Muhamad Sahlan, Tohru Taniguchi, Shigenori Kumazawa

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

We revealed the plant origin of propolis from Lombok, Indonesia; its plant origin was Calophyllum inophyllum Linn. This was confirmed by observing the behavior of stingless bees and from the LC-MS analysis of the 70% ethanol extracts of the propolis and C. inophyllum resin. Furthermore, a new chromanone derivative, calophylloidic acid A (1), was isolated from the stem bark resin of C. inophyllum. The structure of 1 was elucidated using a combination method based on the IR, UV, HRESIMS, and 1D and 2D NMR analyses. The absolute structure of C-2 and C-3 positions of 1 was determined by electronic circular dichroism (ECD). This determination was supported by the time-dependent density functional theory (TD-DFT) and ECD calculations. Additionally, the C. inophyllum resin, propolis, and 1 exhibited significant antibacterial activity against Staphylococcus aureus and Escherichia coli.

Original languageEnglish
Article number100276
JournalResults in Chemistry
Volume4
DOIs
Publication statusPublished - Jan 2022

Keywords

  • Calophylloidic acid A
  • Calophyllum inophyllum
  • Chromanone
  • Indonesia
  • Propolis

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