Nanoparticle synthesis and cytotoxicity of Kaempferia pandurata Roxb. Extract to the growth of MDA-MB-231 breast cancer cell line

Risya Amelia Rahmawanti, Fadilah Fadilah, Brenda Cristie Edina, Lowilius Wiyono, Rafika Indah Paramita

Research output: Contribution to journalArticle

Abstract

Breast cancer is the most common cancer worldwide and in Indonesia. Kaempferia pandurata Roxb. is a herbal plant from South-East Asia which is known for its ability to inhibit the growth of Estrogen Receptor (ER) + breast cancer cell line from the former study. However, its effect on ER- breast cancer cell lines had not been studied. Therefore, we want to examine the cytotoxicity effect of K. pandurata Roxb. on ER- breast cancer cell line (MDA-MB-231). Nanoparticle is a form of preparation that optimizes the activity of any compound to the targeted cell. Therefore, it is expected that it can increase the effectivity of anticancer in Kaempferia pandurata Roxb. In this study, the rhizome of K. pandurata Roxb. trituration was dried and extracted with n-hexane solvent. Nanoparticle of K. pandurata Roxb. was synthesized with CaCl2, chitosan, and alginate by stirring with a magnetic stirrer, adjusting pH, and centrifugation. Then, nanoparticle was analized by UV/VIS spectrofotometry and transmission electron microscopy (TEM). The cytotoxicity of K. pandurata Roxb. extract and nanoparticle were examined with MTT assay. The result of this test is data of inhibition percentage and IC50 value. The result showed that n-hexane extract of K. pandurata Roxb. is synthesized into nanoparticle form with 99,43% yield percentage (entrapment value). Anticancer activity of n-hexane extract and nanoparticle of K. pandurata Roxb. is moderate with IC50 value of the extract is 87,23 μg/ml and the nanoparticle is 24,23 μg/ml. The nanoparticle's activity is better than the extract. n-Hexane extract and nanoparticle of K. pandurata Roxb. has cytotoxicity effects towards MDA-MB-231 cell line. Nanoparticle can increase the cytotoxicity effect of K. pandurata Roxb. extract because its hydrophobic feature and nanometer size.

Original languageEnglish
Pages (from-to)109-114
Number of pages6
JournalPharmacognosy Journal
Volume12
Issue number1
DOIs
Publication statusPublished - 1 Jan 2020

Keywords

  • Breast cancer
  • Kaempferia pandurata Roxb.
  • MDA-MB-231 cells
  • Nanoparticle
  • Temu Kunci

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