TY - JOUR
T1 - Nanocurcumin Potential Effect of SOD Enzyme and Caspase-3 Expression in Lead-Acetate Induced Rats Ovarian Granulosa Cells
AU - Supriyanto, null
AU - I'tishom, Reny
AU - Hendarto, Hendy
AU - Sudjarwo, Sri Agus
AU - Syarifah, Anis Satus
PY - 2021/6
Y1 - 2021/6
N2 - Aim: This study investigated the potential effect of nanocurcumin on the increase of SOD enzyme expression and decrease of caspase-3 in lead acetate-induced rats ovarian granulosa cells.Materials and Methods: Forty five female rats were divided into 5 groups, the negative control group (rats receiving corn oil, one hour later receiving distilled water), positive control group (rats receiving corn oil, one hour later receiving lead acetate of 40 mg/kg bw) and experimental groups 1, 2 and 3 (rats receiving nanocurcumin 50 mg, 100 mg and 200 mg/kg bw). One hour after administering the nanocurcumin, the rats received 40 mg/kg of lead acetate. All groups received oral treatment once a day for 26 days. On day 27 the rats were sacrificed and the expression of SOD and caspase-3 enzymes were measured using immunohistochemical methods.Results: This study found that lead acetate decreased SOD enzyme expression and increased caspase-3. In contrast, nanocurcumin increased SOD enzyme and decreased caspase-3 expression in lead acetate-induced rats ovarian granulosa cells.Conclusion: Nanocurcumin has potential as a strong natural antioxidant by affecting the increase of SOD and the decrease of caspase-3 cells in lead acetate-induced rats ovarian granulosa cells
AB - Aim: This study investigated the potential effect of nanocurcumin on the increase of SOD enzyme expression and decrease of caspase-3 in lead acetate-induced rats ovarian granulosa cells.Materials and Methods: Forty five female rats were divided into 5 groups, the negative control group (rats receiving corn oil, one hour later receiving distilled water), positive control group (rats receiving corn oil, one hour later receiving lead acetate of 40 mg/kg bw) and experimental groups 1, 2 and 3 (rats receiving nanocurcumin 50 mg, 100 mg and 200 mg/kg bw). One hour after administering the nanocurcumin, the rats received 40 mg/kg of lead acetate. All groups received oral treatment once a day for 26 days. On day 27 the rats were sacrificed and the expression of SOD and caspase-3 enzymes were measured using immunohistochemical methods.Results: This study found that lead acetate decreased SOD enzyme expression and increased caspase-3. In contrast, nanocurcumin increased SOD enzyme and decreased caspase-3 expression in lead acetate-induced rats ovarian granulosa cells.Conclusion: Nanocurcumin has potential as a strong natural antioxidant by affecting the increase of SOD and the decrease of caspase-3 cells in lead acetate-induced rats ovarian granulosa cells
KW - Antioxidants; lead acetate; SOD and Caspase-3; nanocurcumin
U2 - 10.37506/ijfmt.v15i2.14650
DO - 10.37506/ijfmt.v15i2.14650
M3 - Article
SN - 0973-9122
JO - Indian Journal of Forensic Medicine and Toxicology
JF - Indian Journal of Forensic Medicine and Toxicology
ER -