The study of nanocellulose has been emerging due to its highly possible applications. The main objective of this research is to fabricate nanocellulose crystalline filament from Sugarcane Bagasse through wet-spinning method. Sugarcane Bagasse is chosen due to its abundance in Indonesia and high cellulose content. In this research, the Sugarcane Bagasse were mechanically treated in order to minimize the size. Secondly, the biomass is pre-treated with NaClO2 (Bleaching) in order to eliminate lignin and hemicellulose. Thirdly, the biomass is treated with Acid Hydrolysis. The variation used in Acid Hydrolysis is HCl with 1%, 3%, and 5%. After obtaining Nanocellulose Crystalline, the biomass was spun through wet-spinning method using 16G needle, 18G needle, 1.5%, and 2% in concentration as the variation. The wet spinning method used a coagulating bath that is filled with Acetone. The characterization used in this research would be Cellulose Content Test, TEM, XRD, and Tensile Strength Test. The average results of this research are 42.75% on cellulose isolation, 63.9% on CNC crystallinity index, 45.3 nm on particle size, and 4.3 lbs. on tensile strength at fracture with 11.91 % on elongation. Nanocellulose Filament used in this research would be applicable for the future textile and material industry that possibly replace fossil fuel-based material.