Mycobacterium leprae deoxyribonucleic acid positivity on skin lesion of untreated leprosy patients and its route to the skin surface

Research output: Contribution to journalArticle

Abstract

The route of transmission of leprosy is still unclear. Multiple opinions exist regarding the environmental dissemination of Mycobacterium leprae (M. leprae). This research aims to identify the positive proportion of M. leprae deoxyribonucleic acid (DNA) on skin lesion surface of untreated multibacillary leprosy patients by polymerase chain reaction (PCR), and its association with the examination result of acid-fast bacilli (AFB) in epidermis, pilosebaceous unit, and sweat glands. The M. leprae DNA sample was taken from smears of the skin lesion surface by using PCR and the skin biopsy test was done to detect AFB in epidermis, pilosebaceous unit, and sweat glands. From 28 subjects, there was 82.1% positive proportion of M. leprae DNA on the skin lesion surface of untreated multi-bacillary leprosy patients, where all were found in the lepromatous leprosy and borderline leprosy type. The result of AFB was positive in epidermis (71.4%), pilosebaceous unit (46.4%) and sweat glands (53.6%). We found M. leprae by using PCR technique on skin lesion surface of untreated multibacillary leprosy patients, and its route which most likely was through the epidermis.

Original languageEnglish
Article number8024
JournalDermatology Reports
Volume11
Issue numberS1
DOIs
Publication statusPublished - 1 Jan 2019

Keywords

  • Deoxyribonucleic acid
  • Mycobacterium leprae
  • Skin structures
  • Skin surface

Fingerprint Dive into the research topics of 'Mycobacterium leprae deoxyribonucleic acid positivity on skin lesion of untreated leprosy patients and its route to the skin surface'. Together they form a unique fingerprint.

  • Cite this