Multiplex PCR for identification of fish substitution in fish fillet products

A. D. Jayaleksana, N. Andayani, A. E. Maryanto

Research output: Contribution to journalConference articlepeer-review


Fish substitution in fish fillet products is a concern in food safety and sustainability. There are many reports about the mislabeling problem in fish fillet products. DNA-based identification plays an important role in food safety and traceability. The use of advanced techniques of barcode using multiplex PCR for species identification substitution from fish fillet products are demonstrated in this study. The primer was designed from three different species that are usually labeled in fish fillet products. Each pair of primers were designed to amplify different PCR products that are specifically recognized as different species. We found 11, 6 and 0 samples labeled as Tilapia, Pangasius, and Snapper respectively. This method is suggested for rapid identification because it is efficient and accurate.

Original languageEnglish
Article number012014
JournalIOP Conference Series: Earth and Environmental Science
Issue number1
Publication statusPublished - 28 Jul 2020
Event3rd Life and Environmental Sciences Academics Forum, LEAF 2019 - Depok, Indonesia
Duration: 11 Jul 2019 → …


  • fillet
  • fish
  • Multiplex
  • substitutions

Fingerprint Dive into the research topics of 'Multiplex PCR for identification of fish substitution in fish fillet products'. Together they form a unique fingerprint.

Cite this