TY - JOUR
T1 - MRNA expression of tumor growth factor-ßeta1 in human glioma
AU - Handoko, H.
AU - Ichwan, S.
AU - Wanandi, S. I.
AU - Hardiany, N. S.
N1 - Funding Information:
This study was funded by Direktorat Riset dan Pengabdian kepada Masyarakat Universitas Indonesia. We thank (DRPM-UI) for accepting this paper to be a part of hibah Publikasi Internasional Terindeks untuk Tugas Akhir Mahasiswa UI (PITTA). The researcher would also like to thank Ecosains Hayati, Indonesia for providing the PCR machine, PCRmax Eco 48.
Publisher Copyright:
© 2018 Institute of Physics Publishing. All rights reserved.
PY - 2018/9/7
Y1 - 2018/9/7
N2 - Glioma describes a tumor that starts within the spinal cord or brain, and originates from glial cells; gliomas are the most commonly-found malignant brain tumors in Indonesia. Until now, there was no specific molecular biomarker used to distinguish gliomas based on tumor grade. TGF-β1 plays a key role in maintaining tissue homeostasis and cancer progression. Because of this, TGF-β1 has potential as a biomarker for differentiating between low-and high-grade gliomas. Samples were collected from patients with gliomas at the Cipto Mangunkusumo Hospital, Indonesia. The samples were categorized as low- (grades 1 and 2) or high- (grades 3 or 4) grade gliomas through histopathologic examination and based on the guidelines set forth by the World Health Organization. Relative mRNA expression of TGF-β1 was quantified through real-time RT-PCR with 18sRNA as the housekeeping gene. There were 27 glioma patient consisting of 17 low-grade glioma tissues and 10 high-grade glioma and 2 normal brain tissues as controls. There were 13 male and 14 female patients, with 63% <40 years old. There was decreased relative expression of TGF-β1 in high-grade, compared to low-grade, gliomas. However, this difference was not statistically significant. The role of TGF-β1 as a definite biomarker was not proven in this study; therefore, more research should be conducted to elaborate the role of TGF-β1 as a biomarker.
AB - Glioma describes a tumor that starts within the spinal cord or brain, and originates from glial cells; gliomas are the most commonly-found malignant brain tumors in Indonesia. Until now, there was no specific molecular biomarker used to distinguish gliomas based on tumor grade. TGF-β1 plays a key role in maintaining tissue homeostasis and cancer progression. Because of this, TGF-β1 has potential as a biomarker for differentiating between low-and high-grade gliomas. Samples were collected from patients with gliomas at the Cipto Mangunkusumo Hospital, Indonesia. The samples were categorized as low- (grades 1 and 2) or high- (grades 3 or 4) grade gliomas through histopathologic examination and based on the guidelines set forth by the World Health Organization. Relative mRNA expression of TGF-β1 was quantified through real-time RT-PCR with 18sRNA as the housekeeping gene. There were 27 glioma patient consisting of 17 low-grade glioma tissues and 10 high-grade glioma and 2 normal brain tissues as controls. There were 13 male and 14 female patients, with 63% <40 years old. There was decreased relative expression of TGF-β1 in high-grade, compared to low-grade, gliomas. However, this difference was not statistically significant. The role of TGF-β1 as a definite biomarker was not proven in this study; therefore, more research should be conducted to elaborate the role of TGF-β1 as a biomarker.
UR - http://www.scopus.com/inward/record.url?scp=85054494181&partnerID=8YFLogxK
U2 - 10.1088/1742-6596/1073/3/032058
DO - 10.1088/1742-6596/1073/3/032058
M3 - Conference article
AN - SCOPUS:85054494181
SN - 1742-6588
VL - 1073
JO - Journal of Physics: Conference Series
JF - Journal of Physics: Conference Series
IS - 3
M1 - 032058
T2 - 2nd Physics and Technologies in Medicine and Dentistry Symposium, PTMDS 2018
Y2 - 18 July 2018 through 18 July 2018
ER -