TY - JOUR
T1 - MRNA expression of tumor growth factor-ßeta1 in human glioma
AU - Handoko, H.
AU - Ichwan, S.
AU - Wanandi, S. I.
AU - Hardiany, N. S.
N1 - Publisher Copyright:
© 2018 Institute of Physics Publishing. All rights reserved.
PY - 2018/9/7
Y1 - 2018/9/7
N2 - Glioma describes a tumor that starts within the spinal cord or brain, and originates from glial cells; gliomas are the most commonly-found malignant brain tumors in Indonesia. Until now, there was no specific molecular biomarker used to distinguish gliomas based on tumor grade. TGF-β1 plays a key role in maintaining tissue homeostasis and cancer progression. Because of this, TGF-β1 has potential as a biomarker for differentiating between low-and high-grade gliomas. Samples were collected from patients with gliomas at the Cipto Mangunkusumo Hospital, Indonesia. The samples were categorized as low- (grades 1 and 2) or high- (grades 3 or 4) grade gliomas through histopathologic examination and based on the guidelines set forth by the World Health Organization. Relative mRNA expression of TGF-β1 was quantified through real-time RT-PCR with 18sRNA as the housekeeping gene. There were 27 glioma patient consisting of 17 low-grade glioma tissues and 10 high-grade glioma and 2 normal brain tissues as controls. There were 13 male and 14 female patients, with 63% <40 years old. There was decreased relative expression of TGF-β1 in high-grade, compared to low-grade, gliomas. However, this difference was not statistically significant. The role of TGF-β1 as a definite biomarker was not proven in this study; therefore, more research should be conducted to elaborate the role of TGF-β1 as a biomarker.
AB - Glioma describes a tumor that starts within the spinal cord or brain, and originates from glial cells; gliomas are the most commonly-found malignant brain tumors in Indonesia. Until now, there was no specific molecular biomarker used to distinguish gliomas based on tumor grade. TGF-β1 plays a key role in maintaining tissue homeostasis and cancer progression. Because of this, TGF-β1 has potential as a biomarker for differentiating between low-and high-grade gliomas. Samples were collected from patients with gliomas at the Cipto Mangunkusumo Hospital, Indonesia. The samples were categorized as low- (grades 1 and 2) or high- (grades 3 or 4) grade gliomas through histopathologic examination and based on the guidelines set forth by the World Health Organization. Relative mRNA expression of TGF-β1 was quantified through real-time RT-PCR with 18sRNA as the housekeeping gene. There were 27 glioma patient consisting of 17 low-grade glioma tissues and 10 high-grade glioma and 2 normal brain tissues as controls. There were 13 male and 14 female patients, with 63% <40 years old. There was decreased relative expression of TGF-β1 in high-grade, compared to low-grade, gliomas. However, this difference was not statistically significant. The role of TGF-β1 as a definite biomarker was not proven in this study; therefore, more research should be conducted to elaborate the role of TGF-β1 as a biomarker.
UR - http://www.scopus.com/inward/record.url?scp=85054494181&partnerID=8YFLogxK
U2 - 10.1088/1742-6596/1073/3/032058
DO - 10.1088/1742-6596/1073/3/032058
M3 - Conference article
AN - SCOPUS:85054494181
SN - 1742-6588
VL - 1073
JO - Journal of Physics: Conference Series
JF - Journal of Physics: Conference Series
IS - 3
M1 - 032058
T2 - 2nd Physics and Technologies in Medicine and Dentistry Symposium, PTMDS 2018
Y2 - 18 July 2018 through 18 July 2018
ER -