Monoclonal antibodies to human IgM

Agus Sjahrurachman; Agustin Indrawaii; Beti Ernawati Dewi; R. Fera Ibrahim; Mardiastuti

doi:10.13181/mji.v8i4.714

Monoclonal antibodies to human IgM

Agus Sjahrurachman, Agustin Indrawaii, Beti Ernawati Dewi, R. Fera Ibrahim, Mardiastuti

Department of Microbiology Clinic

Research output: Contribution to journal › Article › peer-review

Abstract

Availability of specific antihuman IgM antibodies is very important for developing diagnostic kits for infectious diseases that are highly prevalent in Indonesia. In line with such idea, an attempt to construct hybridoma cells producing antihuman IgM antibodies is done by fusing NS-1 myeloma cell line with immunized mouse spleen cells. Hybridomas producing anti human IgM antibodies have been obtained and antibodies produced by several exhaustedly cloned hybridomas has been characterized. Those antibodies are not reactive to human IgG, IgA nor IgG and IgA-deprived human serum and belong to IgG2a subclass.

Original language	English
Pages (from-to)	218-221
Number of pages	4
Journal	Medical Journal of Indonesia
Volume	8
Issue number	4
DOIs	https://doi.org/10.13181/mji.v8i4.714
Publication status	Published - 1 Oct 1999

Keywords

Antihuman IgM
Diagnostic
Monoclonal antibody
Mouse hybridoma

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title = "Monoclonal antibodies to human IgM",

abstract = "Availability of specific antihuman IgM antibodies is very important for developing diagnostic kits for infectious diseases that are highly prevalent in Indonesia. In line with such idea, an attempt to construct hybridoma cells producing antihuman IgM antibodies is done by fusing NS-1 myeloma cell line with immunized mouse spleen cells. Hybridomas producing anti human IgM antibodies have been obtained and antibodies produced by several exhaustedly cloned hybridomas has been characterized. Those antibodies are not reactive to human IgG, IgA nor IgG and IgA-deprived human serum and belong to IgG2a subclass.",

keywords = "Antihuman IgM, Diagnostic, Monoclonal antibody, Mouse hybridoma",

author = "Agus Sjahrurachman and Agustin Indrawaii and Dewi, {Beti Ernawati} and Ibrahim, {R. Fera} and Mardiastuti",

year = "1999",

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doi = "10.13181/mji.v8i4.714",

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T1 - Monoclonal antibodies to human IgM

AU - Sjahrurachman, Agus

AU - Indrawaii, Agustin

AU - Dewi, Beti Ernawati

AU - Ibrahim, R. Fera

AU - Mardiastuti,

PY - 1999/10/1

Y1 - 1999/10/1

N2 - Availability of specific antihuman IgM antibodies is very important for developing diagnostic kits for infectious diseases that are highly prevalent in Indonesia. In line with such idea, an attempt to construct hybridoma cells producing antihuman IgM antibodies is done by fusing NS-1 myeloma cell line with immunized mouse spleen cells. Hybridomas producing anti human IgM antibodies have been obtained and antibodies produced by several exhaustedly cloned hybridomas has been characterized. Those antibodies are not reactive to human IgG, IgA nor IgG and IgA-deprived human serum and belong to IgG2a subclass.

AB - Availability of specific antihuman IgM antibodies is very important for developing diagnostic kits for infectious diseases that are highly prevalent in Indonesia. In line with such idea, an attempt to construct hybridoma cells producing antihuman IgM antibodies is done by fusing NS-1 myeloma cell line with immunized mouse spleen cells. Hybridomas producing anti human IgM antibodies have been obtained and antibodies produced by several exhaustedly cloned hybridomas has been characterized. Those antibodies are not reactive to human IgG, IgA nor IgG and IgA-deprived human serum and belong to IgG2a subclass.

KW - Antihuman IgM

KW - Diagnostic

KW - Monoclonal antibody

KW - Mouse hybridoma

UR - http://www.scopus.com/inward/record.url?scp=85008689627&partnerID=8YFLogxK

U2 - 10.13181/mji.v8i4.714

DO - 10.13181/mji.v8i4.714

M3 - Article

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SN - 0853-1773

VL - 8

SP - 218

EP - 221

JO - Medical Journal of Indonesia

JF - Medical Journal of Indonesia

IS - 4

ER -

Monoclonal antibodies to human IgM

Abstract

Keywords

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