Molecular phylogenetic analyses of filamentous fungi from deteriorated old Chinese manuscripts in Central Library Universitas Indonesia

M. K. Rachmania, A. Oetari, M. Rahmadewi, W. Sjamsuridzal

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Phylogenetic analyses can be used in order to obtain the evolutionary relationship and evolutionary distances among the species of fungi. Molecular phylogenetic analyses of ITS regions (ITS1; 5.8S rDNA; ITS2) of ribosomal DNA of filamentous fungi from four deteriorated old Chinese manuscripts in Central Library Universitas Indonesia was conducted to obtain information of the phylogenetic relationships among the species, closely related species, species diversity, and taxonomic position. Phylogenetic tree construction from the ITS regions was performed using methods of Neighbor-Joining, Maximum Likelihood, and Minimum Evolution with Kimura's two-parameter model, and bootstrap 1,000 replicates. Eleven fungal strains belonged to three classes (Dothideomycetes, Eurotiomycetes and Sordariomycetes), five orders (Capnodiales, Eurotiales, Hypocreales, Pleosporales, and Xylariales), six families (Aspergillaceae, Cladosporiaceae, Microdochiaceae, Ophiocordycipitaceae, Pleosporaceae, and Trichocomaceae), six genera (Aspergillus, Cladosporium, Curvularia, Penicillium, Purpureocillium, and Sarocladium), and three closely related species (Aspergillus pseudodeflectus, Penicillium coffeae, and Purpureocillium lilacinum syn. Paecilomyces lilacinus). The ITS sequences of other strains were not identical to any type strains. Further studies using multiple locus analyses are required to confirm the species identities of these strains.

Original languageEnglish
Title of host publicationProceedings of the 3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
EditorsRatna Yuniati, Terry Mart, Ivandini T. Anggraningrum, Djoko Triyono, Kiki A. Sugeng
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735417410
DOIs
Publication statusPublished - 22 Oct 2018
Event3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017 - Bali, Indonesia
Duration: 26 Jul 201727 Jul 2017

Publication series

NameAIP Conference Proceedings
Volume2023
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
Country/TerritoryIndonesia
CityBali
Period26/07/1727/07/17

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