Molecular Detection and Genotyping of Active Toxoplasmosis from Blood Sample

Toxoplasma gondii, which is an obligate intracellular protozoan, and its primer infection during pregnancy can cause several problems. This study presents a case report of a woman who delivered a baby with multi-organ failure and was diagnosed with congenital tox-oplasmosis. During the gestation period, there was no history of febrile, enlargement of lymph nodes, or common cold symptoms, but there was a history of miscarriage. The serology test of anti-Toxoplasma im-munoglobulin (Ig)G and IgM were reactive. The IgG avidity test was 90%, and positive SAG2. The sequencing and analysis of polymerase chain reaction product assumed to have the Type I T. gondii, which must be confirmed using other loci apart from SAG2. This case report aims to raise awareness of the risk of congenital toxoplasmosis in asymptomatic mothers with a history of miscarriage. Routine and serial screening for the disease is recommended in high-risk pregnant women to prevent congenital transmission.