Detection and genotyping of group A rotavirus strains from stool samples in young children with diarrhea in Indonesia were examined using reverse transcription-nested multiplex PCR. Of 421 stool specimens, 257 samples was rotavirus positive. G1 type was the most common G-type (54%), followed by G2 (6%) and G9 (3%). P was the most common P-type (39%), followed by P (19%), P (10%) and P 1%. Eighteen percent of the samples had mixed G genotype infection and 5% had mixed P genotype infection. The prevalence of G-P combination type was genotype G1P (24%), followed by G1P (7%), G2P (3%), and G1P (2%). A total of 118 specimens could not be assigned as a G and/or P type suggesting the presence of new circulating genotypes in Indonesia.
|Number of pages||6|
|Journal||Southeast Asian Journal of Tropical Medicine and Public Health|
|Publication status||Published - 1 Mar 2010|
- Multiplex nested PCR
- Reverse transcription
- Rotavirus A