Molecular characterization of human group a rotavirus from stool samples in young children with diarrhea in Indonesia

Maksum Radji, Shannon D. Putman, Amarila Malik, Refda Husrima, Erlin Listyaningsih

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9 Citations (Scopus)

Abstract

Detection and genotyping of group A rotavirus strains from stool samples in young children with diarrhea in Indonesia were examined using reverse transcription-nested multiplex PCR. Of 421 stool specimens, 257 samples was rotavirus positive. G1 type was the most common G-type (54%), followed by G2 (6%) and G9 (3%). P[8] was the most common P-type (39%), followed by P[6] (19%), P[4] (10%) and P[11] 1%. Eighteen percent of the samples had mixed G genotype infection and 5% had mixed P genotype infection. The prevalence of G-P combination type was genotype G1P[8] (24%), followed by G1P[6] (7%), G2P[4] (3%), and G1P[4] (2%). A total of 118 specimens could not be assigned as a G and/or P type suggesting the presence of new circulating genotypes in Indonesia.

Original languageEnglish
Pages (from-to)341-346
Number of pages6
JournalSoutheast Asian Journal of Tropical Medicine and Public Health
Volume41
Issue number2
Publication statusPublished - 1 Mar 2010

Keywords

  • Diarrhea
  • Multiplex nested PCR
  • Reverse transcription
  • Rotavirus A
  • VP4
  • VP7

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