Modification of nitrogen-terminated boron-doped diamond electrodes with gold nanoparticles and hemoglobin for acrylamide biosensors

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Boron-doped diamond (BDD) was modified with gold nanoparticles (AuNPs) and hemoglobin (Hb) through nitrogen groups on the surface of BDD to produce an electrode with good selectivity, sensitivity, and affinity to be applied as an acrylamide biosensor. Prior to modification with Hb, the BDD was modified with AuNPs to increase the affinity of BDD with nitrogen termination (N-BDD) towards Hb. The electrochemical behavior of the hemoglobin-modified with gold nanoparticles on the surface of N-BDD electrode (Hb-AuNPs-N-BDD) in the presence of acrylamide was studied in comparison to hemoglobin-modified gold electrodes (Hb-Au). Cyclic voltammetry indicated the optimum concentration of Hb was obtained at 0.6 mg/mL in Hb-Au electrode and 0.02 mg/mL in Hb-AuNPs-N-BDD electrode. The responses were linear against the acrylamide concentration range of 0 - 30 μM with an estimated LOD of 38.15 μM at Hb-Au electrode and 13.10 μM at Hb-AuNPs-BDDN electrode. The results indicated that Hb-AuNPs-N-BDD electrode has a better performance than Hb-Au electrode as the acrylamide sensors.

Original languageEnglish
Title of host publicationProceedings of the 3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
EditorsRatna Yuniati, Terry Mart, Ivandini T. Anggraningrum, Djoko Triyono, Kiki A. Sugeng
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735417410
DOIs
Publication statusPublished - 22 Oct 2018
Event3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017 - Bali, Indonesia
Duration: 26 Jul 201727 Jul 2017

Publication series

NameAIP Conference Proceedings
Volume2023
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
CountryIndonesia
CityBali
Period26/07/1727/07/17

Keywords

  • Acrylamide
  • Biosensor
  • Boron-Doped Diamond
  • Gold Nanoparticles
  • Hemoglobin

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    Annisa, T. N., Saefudin, E., & Anggraningrum, I. T. (2018). Modification of nitrogen-terminated boron-doped diamond electrodes with gold nanoparticles and hemoglobin for acrylamide biosensors. In R. Yuniati, T. Mart, I. T. Anggraningrum, D. Triyono, & K. A. Sugeng (Eds.), Proceedings of the 3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017 [020108] (AIP Conference Proceedings; Vol. 2023). American Institute of Physics Inc.. https://doi.org/10.1063/1.5064105