Modification of boron-doped diamond with gold through wet-chemical seeding and electrodeposition techniques for the application of acrylamide biosensor

Acrylamide is known as carcinogenic and neurotoxin substrates. An alternative method for acrylamide detection is by using hemoglobin-based biosensors as it can provide a simple, rapid, and sensitive method. In this research, the electrodes for biosensor of acrylamide were prepared by modifying boron-doped diamond (BDD) with gold and hemoglobin (Hb). Wet-chemical seeding followed by electrochemical overgrowth of seeds technique was performed to modify the BDD electrodes with gold. The characterization with Scanning Electron Microscopy-Energy Dispersive Spectroscopy X-Ray (SEM-EDS) showed the gold coverage of 12.74 % on the BDD surface. The gold-modified BDD was then modified with 0.25 mM Hb. The prepared sensor can be successfully applied in the concentration range of acrylamide from 0.6 to 6 μM with high linearity (R² = 0.9901) and an estimated limit of detection of 0.845 μM.