Microsphere molecularly imprinted solid-phase extraction for diazepam analysis using itaconic acid as a monomer in propanol

Aliya Nur Hasanah, Ike Susanti, Marcellino Marcellino, Gabriella Josephine Maranata, Febrina Amelia Saputri, Rimadani Pratiwi

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)


Diazepam (DZP) is a benzodiazepine drug used as an anti-drug and sedative. It is often misused to induce or create euphoria in combination with other drugs (high or fly sensation) or administered alone. So far, screening for DZP abuse with sensitive analytical methods is needed, as its small concentrations make it difficult to detect. Increased sensitivity of the analytical method can be obtained by using a preparation method that selectively separates the analyte from the sample matrix. Molecularly imprinted polymer (MIP) is one of the preparation solutions with good selectivity, specificity, and sensitivity. MIP was made from DZP as a template, itaconic acid, and ethylene glycol dimethacrylate in a composition of 1:4:20. MIP was made by precipitation polymerisation to obtain microsphere polymer type. MIP had a binding capacity value of 0.0557 mg/g and followed the Freundlich isotherm. Application of the microsphere MIP on spiked blood serum resulted in a recovery of 105.63 ± 1.0% for MIP compared to 21.28 ± 0.4% for non-imprinted polymer, with the imprinting factor value reaching 4.96. Hence, MIP DZP with itaconic acid as a functional monomer and propanol as a porogen, fabricated by the precipitation polymerisation method, is a promising sorbent for DZP extraction in biological fluids.

Original languageEnglish
Pages (from-to)604-613
Number of pages10
JournalOpen Chemistry
Issue number1
Publication statusPublished - 1 Jan 2021


  • diazepam
  • itaconic acid
  • molecularly imprinted solid-phase extraction
  • precipitation polymerisation
  • propanol
  • solid-phase extraction


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