TY - JOUR
T1 - Microsphere molecularly imprinted solid-phase extraction for diazepam analysis using itaconic acid as a monomer in propanol
AU - Hasanah, Aliya Nur
AU - Susanti, Ike
AU - Marcellino, Marcellino
AU - Maranata, Gabriella Josephine
AU - Saputri, Febrina Amelia
AU - Pratiwi, Rimadani
N1 - Funding Information:
Funding information: This research was funded by the Ministry of Research and Higher Education of the Republic of Indonesia, grant number 1827/UN6.3.1/LT/2020, and the APC was funded by Universitas Padjadjaran.
Publisher Copyright:
© 2021 Aliya Nur Hasanah et al., published by De Gruyter.
PY - 2021/1/1
Y1 - 2021/1/1
N2 - Diazepam (DZP) is a benzodiazepine drug used as an anti-drug and sedative. It is often misused to induce or create euphoria in combination with other drugs (high or fly sensation) or administered alone. So far, screening for DZP abuse with sensitive analytical methods is needed, as its small concentrations make it difficult to detect. Increased sensitivity of the analytical method can be obtained by using a preparation method that selectively separates the analyte from the sample matrix. Molecularly imprinted polymer (MIP) is one of the preparation solutions with good selectivity, specificity, and sensitivity. MIP was made from DZP as a template, itaconic acid, and ethylene glycol dimethacrylate in a composition of 1:4:20. MIP was made by precipitation polymerisation to obtain microsphere polymer type. MIP had a binding capacity value of 0.0557 mg/g and followed the Freundlich isotherm. Application of the microsphere MIP on spiked blood serum resulted in a recovery of 105.63 ± 1.0% for MIP compared to 21.28 ± 0.4% for non-imprinted polymer, with the imprinting factor value reaching 4.96. Hence, MIP DZP with itaconic acid as a functional monomer and propanol as a porogen, fabricated by the precipitation polymerisation method, is a promising sorbent for DZP extraction in biological fluids.
AB - Diazepam (DZP) is a benzodiazepine drug used as an anti-drug and sedative. It is often misused to induce or create euphoria in combination with other drugs (high or fly sensation) or administered alone. So far, screening for DZP abuse with sensitive analytical methods is needed, as its small concentrations make it difficult to detect. Increased sensitivity of the analytical method can be obtained by using a preparation method that selectively separates the analyte from the sample matrix. Molecularly imprinted polymer (MIP) is one of the preparation solutions with good selectivity, specificity, and sensitivity. MIP was made from DZP as a template, itaconic acid, and ethylene glycol dimethacrylate in a composition of 1:4:20. MIP was made by precipitation polymerisation to obtain microsphere polymer type. MIP had a binding capacity value of 0.0557 mg/g and followed the Freundlich isotherm. Application of the microsphere MIP on spiked blood serum resulted in a recovery of 105.63 ± 1.0% for MIP compared to 21.28 ± 0.4% for non-imprinted polymer, with the imprinting factor value reaching 4.96. Hence, MIP DZP with itaconic acid as a functional monomer and propanol as a porogen, fabricated by the precipitation polymerisation method, is a promising sorbent for DZP extraction in biological fluids.
KW - diazepam
KW - itaconic acid
KW - molecularly imprinted solid-phase extraction
KW - precipitation polymerisation
KW - propanol
KW - solid-phase extraction
UR - http://www.scopus.com/inward/record.url?scp=85106574841&partnerID=8YFLogxK
U2 - 10.1515/chem-2021-0050
DO - 10.1515/chem-2021-0050
M3 - Article
AN - SCOPUS:85106574841
VL - 19
SP - 604
EP - 613
JO - Open Chemistry
JF - Open Chemistry
SN - 2391-5420
IS - 1
ER -