TY - JOUR
T1 - Microscopical Evaluation and TLC Analysis of Pluchea indica (L.) Less
T2 - Leaf, Stem, and Root
AU - Hikmawanti, Ni Putu Ermi
AU - Saputri, Fadlina Chany
AU - Yanuar, Arry
AU - Ningrum, Ratih Asmana
AU - Mun’im, Abdul
AU - Hayati, Hayati
N1 - Publisher Copyright:
© 2024, Bogor Agricultural University. All rights reserved.
PY - 2024/1
Y1 - 2024/1
N2 - Pluchea indica (L.) Less is traditionally utilized to treat postpartum women in Indonesia. The plant has many pharmacological properties, so that it can be further developed as herbal medicine. In that development process, plant authentication is needed to ensure the quality of raw materials. A simple microscopical and thin-layer chromatography (TLC) analysis might be a way to authenticate the plant, but it has never been reported. So, this study evaluates the microscopical and TLC analysis for P. indica authentication in standardized herbal medicines production. Plant microscopic observation, fluorescence analysis, and polyphenol screening were conducted. n-Hexane, ethyl acetate, and methanol extracts of plant organs were then analyzed by TLC. Here, we reported that in microscopical analysis the simplicia of P. indica contains trichomes and tannin-containing cells. In addition, chlorogenic acid as a marker was present in TLC analysis by ethyl acetate-water-formic acid-acetic acid (8.5:1.5:1:1, v/v). The results of this evaluation might provide additional information in the identification, authentication, and quality control of P. indica as a raw material for herbal medicine.
AB - Pluchea indica (L.) Less is traditionally utilized to treat postpartum women in Indonesia. The plant has many pharmacological properties, so that it can be further developed as herbal medicine. In that development process, plant authentication is needed to ensure the quality of raw materials. A simple microscopical and thin-layer chromatography (TLC) analysis might be a way to authenticate the plant, but it has never been reported. So, this study evaluates the microscopical and TLC analysis for P. indica authentication in standardized herbal medicines production. Plant microscopic observation, fluorescence analysis, and polyphenol screening were conducted. n-Hexane, ethyl acetate, and methanol extracts of plant organs were then analyzed by TLC. Here, we reported that in microscopical analysis the simplicia of P. indica contains trichomes and tannin-containing cells. In addition, chlorogenic acid as a marker was present in TLC analysis by ethyl acetate-water-formic acid-acetic acid (8.5:1.5:1:1, v/v). The results of this evaluation might provide additional information in the identification, authentication, and quality control of P. indica as a raw material for herbal medicine.
KW - anatomy
KW - beluntas
KW - herbal drugs
KW - phenolic
KW - quality control
KW - TLC
UR - http://www.scopus.com/inward/record.url?scp=85176558461&partnerID=8YFLogxK
U2 - 10.4308/hjb.31.1.71-81
DO - 10.4308/hjb.31.1.71-81
M3 - Article
AN - SCOPUS:85176558461
SN - 1978-3019
VL - 31
SP - 71
EP - 81
JO - HAYATI Journal of Biosciences
JF - HAYATI Journal of Biosciences
IS - 1
ER -