Microbial biofilms on facial prostheses

Nina Ariani; Arjan Vissink; Robert P. van Oort; Lindawati Kusdhany; Ariadna Djais; Tri Budi W. Rahardjo; Henny C. van der Mei; Bastiaan P. Krom

doi:10.1080/08927014.2012.698614

Microbial biofilms on facial prostheses

Nina Ariani, Arjan Vissink, Robert P. van Oort, Lindawati Kusdhany, Ariadna Djais, Tri Budi W. Rahardjo, Henny C. van der Mei, Bastiaan P. Krom

Research output: Contribution to journal › Article › peer-review

41 Citations (Scopus)

Abstract

The composition of microbial biofilms on silicone rubber facial prostheses was investigated and compared with the microbial flora on healthy and prosthesis-covered skin. Scanning electron microscopy showed the presence of mixed bacterial and yeast biofilms on and deterioration of the surface of the prostheses. Microbial culturing confirmed the presence of yeasts and bacteria. Microbial colonization was significantly increased on prosthesis-covered skin compared to healthy skin. Candida spp. were exclusively isolated from prosthesis-covered skin and from prostheses. Biofilms from prostheses showed the least diverse band-profile in denaturing gradient gel electrophoresis (DGGE) whereas prosthesis-covered skin showed the most diverse band-profile. Bacterial diversity exceeded yeast diversity in all samples. It is concluded that occlusion of the skin by prostheses creates a favorable niche for opportunistic pathogens such as Candida spp. and Staphylococcus aureus. Biofilms on healthy skin, skin underneath the prosthesis and on the prosthesis had a comparable composition, but the numbers present differed according to the microorganism.

Original language	English
Pages (from-to)	583-591
Number of pages	9
Journal	Biofouling
Volume	28
Issue number	6
DOIs	https://doi.org/10.1080/08927014.2012.698614
Publication status	Published - Jul 2012

Keywords

facial prosthesis
microbial biofilms
microbial composition
silicone rubber degradation

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10.1080/08927014.2012.698614

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@article{d65268d903054b508bc2c2fe9497a9d8,

title = "Microbial biofilms on facial prostheses",

abstract = "The composition of microbial biofilms on silicone rubber facial prostheses was investigated and compared with the microbial flora on healthy and prosthesis-covered skin. Scanning electron microscopy showed the presence of mixed bacterial and yeast biofilms on and deterioration of the surface of the prostheses. Microbial culturing confirmed the presence of yeasts and bacteria. Microbial colonization was significantly increased on prosthesis-covered skin compared to healthy skin. Candida spp. were exclusively isolated from prosthesis-covered skin and from prostheses. Biofilms from prostheses showed the least diverse band-profile in denaturing gradient gel electrophoresis (DGGE) whereas prosthesis-covered skin showed the most diverse band-profile. Bacterial diversity exceeded yeast diversity in all samples. It is concluded that occlusion of the skin by prostheses creates a favorable niche for opportunistic pathogens such as Candida spp. and Staphylococcus aureus. Biofilms on healthy skin, skin underneath the prosthesis and on the prosthesis had a comparable composition, but the numbers present differed according to the microorganism.",

keywords = "facial prosthesis, microbial biofilms, microbial composition, silicone rubber degradation",

author = "Nina Ariani and Arjan Vissink and {van Oort}, {Robert P.} and Lindawati Kusdhany and Ariadna Djais and Rahardjo, {Tri Budi W.} and {van der Mei}, {Henny C.} and Krom, {Bastiaan P.}",

note = "Funding Information: This study was supported by a Bernouilli Foundation Grant from the University of Groningen, sandwich program University of Indonesia and UMCG, and an International Society for Maxillofacial Rehabilitation (ISMR) research award 2008.",

year = "2012",

month = jul,

doi = "10.1080/08927014.2012.698614",

language = "English",

volume = "28",

pages = "583--591",

journal = "Biofouling",

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AU - Ariani, Nina

AU - Vissink, Arjan

AU - van Oort, Robert P.

AU - Kusdhany, Lindawati

AU - Djais, Ariadna

AU - Rahardjo, Tri Budi W.

AU - van der Mei, Henny C.

AU - Krom, Bastiaan P.

N1 - Funding Information: This study was supported by a Bernouilli Foundation Grant from the University of Groningen, sandwich program University of Indonesia and UMCG, and an International Society for Maxillofacial Rehabilitation (ISMR) research award 2008.

PY - 2012/7

Y1 - 2012/7

N2 - The composition of microbial biofilms on silicone rubber facial prostheses was investigated and compared with the microbial flora on healthy and prosthesis-covered skin. Scanning electron microscopy showed the presence of mixed bacterial and yeast biofilms on and deterioration of the surface of the prostheses. Microbial culturing confirmed the presence of yeasts and bacteria. Microbial colonization was significantly increased on prosthesis-covered skin compared to healthy skin. Candida spp. were exclusively isolated from prosthesis-covered skin and from prostheses. Biofilms from prostheses showed the least diverse band-profile in denaturing gradient gel electrophoresis (DGGE) whereas prosthesis-covered skin showed the most diverse band-profile. Bacterial diversity exceeded yeast diversity in all samples. It is concluded that occlusion of the skin by prostheses creates a favorable niche for opportunistic pathogens such as Candida spp. and Staphylococcus aureus. Biofilms on healthy skin, skin underneath the prosthesis and on the prosthesis had a comparable composition, but the numbers present differed according to the microorganism.

AB - The composition of microbial biofilms on silicone rubber facial prostheses was investigated and compared with the microbial flora on healthy and prosthesis-covered skin. Scanning electron microscopy showed the presence of mixed bacterial and yeast biofilms on and deterioration of the surface of the prostheses. Microbial culturing confirmed the presence of yeasts and bacteria. Microbial colonization was significantly increased on prosthesis-covered skin compared to healthy skin. Candida spp. were exclusively isolated from prosthesis-covered skin and from prostheses. Biofilms from prostheses showed the least diverse band-profile in denaturing gradient gel electrophoresis (DGGE) whereas prosthesis-covered skin showed the most diverse band-profile. Bacterial diversity exceeded yeast diversity in all samples. It is concluded that occlusion of the skin by prostheses creates a favorable niche for opportunistic pathogens such as Candida spp. and Staphylococcus aureus. Biofilms on healthy skin, skin underneath the prosthesis and on the prosthesis had a comparable composition, but the numbers present differed according to the microorganism.

KW - facial prosthesis

KW - microbial biofilms

KW - microbial composition

KW - silicone rubber degradation

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DO - 10.1080/08927014.2012.698614

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Microbial biofilms on facial prostheses

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