TY - JOUR
T1 - Micro-band Boron-doped Diamond Electrode in Capillary Electrophoresis for Simultaneous Detection of AMP, ADP, and ATP
AU - Prayikaputri, Putu Udiyani
AU - Jiwanti, Prastika Krisma
AU - Nasution, Mochammad Arfin Fardiansyah
AU - Gunlazuardi, Jarnuzi
AU - Saepudin, Endang
AU - Einaga, Yasuaki
AU - Ivandini, Tribidasari Anggraningrum
N1 - Funding Information:
The authors would like to thank the Ministry of Research and Technology, Republic of Indonesia, for the financial support given to this research through Hibah Penelitian Dasar Unggulan Perguruan Tinggi (Grant No.: 1600/UN2.R3.1/HKP.05.00/2019). The authors also declare that there is no conflict of interest regarding the publication of this article.
Publisher Copyright:
© 2021, International Journal of Technology. All Rights Reserved.
PY - 2021
Y1 - 2021
N2 - A micro-band boron-doped diamond (BDD) electrode was prepared by sealing a piece of BDD film with an area of 1.11×10-7 m2 between two insulating plates, one Teflon and one silicon rubber, to form sandwich-like layers, so the surface area could be investigated. The micro-band BDD was combined with capillary zone electrophoresis as an electrode for the simultaneous detection of adenosine monophosphate (AMP), adenosine diphosphate (ADP), and adenosine triphosphate (ATP) in a solution. These adenosine phosphates can be separated with a 0.3 m-long fused silica capillary using Britton–Robinson buffers at pH 2.0. Current in the concentration range of 0.1 to 2.0 mM were linear with the limits of detection of 0.004 μM, 0.006 μM, and 0.011 μM for AMP, ADP, and ATP, respectively. A comparison with an unmodified BDD as the detector in the same electrophoresis system showed that the micro-band generated better limits of detection (LODs) than the macroelectrode. This method was successfully applied to human urine samples injected with three adenosine phosphates, as well as adenine and guanine, which can be well-separated with recovery percentages of adenine, guanine, AMP, ADP, and ATP of 99.2%, 102.5%, 107.4%, 107.7%, and 105.4%, respectively.
AB - A micro-band boron-doped diamond (BDD) electrode was prepared by sealing a piece of BDD film with an area of 1.11×10-7 m2 between two insulating plates, one Teflon and one silicon rubber, to form sandwich-like layers, so the surface area could be investigated. The micro-band BDD was combined with capillary zone electrophoresis as an electrode for the simultaneous detection of adenosine monophosphate (AMP), adenosine diphosphate (ADP), and adenosine triphosphate (ATP) in a solution. These adenosine phosphates can be separated with a 0.3 m-long fused silica capillary using Britton–Robinson buffers at pH 2.0. Current in the concentration range of 0.1 to 2.0 mM were linear with the limits of detection of 0.004 μM, 0.006 μM, and 0.011 μM for AMP, ADP, and ATP, respectively. A comparison with an unmodified BDD as the detector in the same electrophoresis system showed that the micro-band generated better limits of detection (LODs) than the macroelectrode. This method was successfully applied to human urine samples injected with three adenosine phosphates, as well as adenine and guanine, which can be well-separated with recovery percentages of adenine, guanine, AMP, ADP, and ATP of 99.2%, 102.5%, 107.4%, 107.7%, and 105.4%, respectively.
KW - Adenosine phosphates
KW - Boron-doped diamond
KW - Capillary zone electrophoresis
KW - Electrochemical detection
KW - Micro-band electrode
UR - http://www.scopus.com/inward/record.url?scp=85110251200&partnerID=8YFLogxK
U2 - 10.14716/ijtech.v12i2.4368
DO - 10.14716/ijtech.v12i2.4368
M3 - Article
AN - SCOPUS:85110251200
SN - 2086-9614
VL - 12
SP - 252
EP - 262
JO - International Journal of Technology
JF - International Journal of Technology
IS - 2
ER -