The advancement of molecular biology provides benefit in the field of medicine to develop gene therapy. The aim of gene therapy is to repair the genetic damage or to replace damaged gene with the normal gene. Delivery of gene is carried out by transfection technique, a technique to transfer nucleic acid into eukaryote cells either using viral vectors (known as transduction), and also using non viral method such as chemical substance, lipid and physical method. Some of the viral vectors used in the transduction are retrovirus, adenovirus, Adeno-associated virus (AAV) and Herpes Simplex Virus (HSV). The success of transfection is determined by various factors which can be assessed using several reporters such as Green Fluorescence Protein (GFP).
- gene therapy, non viral transfection, transduction, viral vector.