TY - JOUR
T1 - Methylation analysis of plasminogen activator inhibitor-1 (PAI-1) gene in ovarian and peritoneal endometriosis
AU - Kinasih, T.
AU - Febri, R. R.
AU - Annisa, N. G.
AU - Natadisastra, R. M.
AU - Asmarinah,
N1 - Publisher Copyright:
© 2018 Institute of Physics Publishing. All rights reserved.
PY - 2018/9/7
Y1 - 2018/9/7
N2 - Endometriosis, defined as the growth of endometrial-like tissue outside the uterus, leads to the emergence of chronic inflammatory reactions, which are influenced by the plasminogen activator systems that are known to play a role in fibrinolysis. Hypofibrinolysis due to the excessive expression of the plasminogen activator inhibitor-1 (PAI-1) gene occurs in endometriotic cells. It has been known that the PAI-1 level is lower in normal endometrial cells than in endometriotic cells. The aim of this study is to assess the methylation level of PAI-1 in association with ovarian and peritoneal endometriosis tissues. This was a comparative cross-sectional study conducted on 13 women with ovarian endometriosis, 5 with peritoneal endometriosis, and 8 without endometriosis. DNA from the patient samples was isolated and subjected to bisulfite conversion. DNA methylation was observed by performing the methylation-specific polymerase chain reaction (MSP) method, followed by electrophoresis. The methylation level of PAI-1 was determined by measuring the band intensity using the ImageJ software. The results were statistically analyzed using the Kruskal-Wallis test. A twotailed p value of <0.05 was considered to be statistically significant. Statistically significant difference was noted in the methylation levels of PAI-1 in ovarian endometriosis and peritoneal endometriosis samples compared with those noted in control samples (p = 0.006 and p = 0.003, respectively). The methylation levels in both sample types were lower than those in the control samples. However, the difference between the methylation levels of PAI-1 in peritoneal endometriosis and ovarian endometriosis were not statistically significant (p > 0.05). We found a low methylation level in the promoter region of PAI-1 gene, which led to an increase in the gene expression that may contribute as a risk factor in ovarian endometriosis and peritoneum endometriosis.
AB - Endometriosis, defined as the growth of endometrial-like tissue outside the uterus, leads to the emergence of chronic inflammatory reactions, which are influenced by the plasminogen activator systems that are known to play a role in fibrinolysis. Hypofibrinolysis due to the excessive expression of the plasminogen activator inhibitor-1 (PAI-1) gene occurs in endometriotic cells. It has been known that the PAI-1 level is lower in normal endometrial cells than in endometriotic cells. The aim of this study is to assess the methylation level of PAI-1 in association with ovarian and peritoneal endometriosis tissues. This was a comparative cross-sectional study conducted on 13 women with ovarian endometriosis, 5 with peritoneal endometriosis, and 8 without endometriosis. DNA from the patient samples was isolated and subjected to bisulfite conversion. DNA methylation was observed by performing the methylation-specific polymerase chain reaction (MSP) method, followed by electrophoresis. The methylation level of PAI-1 was determined by measuring the band intensity using the ImageJ software. The results were statistically analyzed using the Kruskal-Wallis test. A twotailed p value of <0.05 was considered to be statistically significant. Statistically significant difference was noted in the methylation levels of PAI-1 in ovarian endometriosis and peritoneal endometriosis samples compared with those noted in control samples (p = 0.006 and p = 0.003, respectively). The methylation levels in both sample types were lower than those in the control samples. However, the difference between the methylation levels of PAI-1 in peritoneal endometriosis and ovarian endometriosis were not statistically significant (p > 0.05). We found a low methylation level in the promoter region of PAI-1 gene, which led to an increase in the gene expression that may contribute as a risk factor in ovarian endometriosis and peritoneum endometriosis.
UR - http://www.scopus.com/inward/record.url?scp=85054519690&partnerID=8YFLogxK
U2 - 10.1088/1742-6596/1073/3/032081
DO - 10.1088/1742-6596/1073/3/032081
M3 - Conference article
AN - SCOPUS:85054519690
SN - 1742-6588
VL - 1073
JO - Journal of Physics: Conference Series
JF - Journal of Physics: Conference Series
IS - 3
M1 - 032081
T2 - 2nd Physics and Technologies in Medicine and Dentistry Symposium, PTMDS 2018
Y2 - 18 July 2018 through 18 July 2018
ER -