TY - JOUR
T1 - Metabolic and immune response to high-fat diet in healthy urban Indonesian males with family history of type 2 diabetes mellitus
AU - Budiyati, Akterono
AU - Purnamasari, Dyah
AU - Wibowo, Heri
AU - Widyahening, Indah Suci
AU - Soewondo, Pradana
N1 - Funding Information:
Funding: This study was supported by Direktorat Jenderal Pendidikan Tinggi Republik Indonesia under Penelitian Dasar Unggulan Perguruan Tinggi program in term 2019-2020 with grant number 173/SP2H/LT/ DRPM/2019.
Publisher Copyright:
© 2023, Lab and Life Press. All rights reserved.
PY - 2023
Y1 - 2023
N2 - Objectives: Non-diabetic first-degree relatives (FDR) of type 2 diabetes mellitus (T2DM) patients have been reported to have relatively higher insulin resistance and inflammatory markers compared to population without family history of T2DM. We investigated whether healthy FDR T2DM of Indonesian males living in urban area are more susceptible to the adverse effects of high-fat diet (HFD) than non-FDR subjects. Methods: Twenty-seven normoglycemic and normotensive FDR males and 28 age-and-body-mass-index-(BMI)-matched healthy non-FDR males underwent a 5-days HFD challenge. Dietary intake before and after HFD were collected by 24-hours food recall. Metabolic profiles and plasma cytokine levels were assessed before and after the HFD intervention. Results: Within similar BMI profile between groups, FDR subjects showed significantly bigger waist circumference (p=0.001) and higher triglyceride (p=0,03) than those of non-FDR. Despite similar HOMA-IR and IL-6 responses to 5-days HFD, significant increase of plasma TNF-α/IL-10 ratio found in FDR subjects, while in contrary, TNF-α/IL-10 ratio significantly decreased in non-FDR group (p<0.001), resulting an OR of 7.1 (95% CI 2.2-23.4) for FDR to develop elevated plasma TNF-α/IL-10 ratio in response to HFD. The tendency was as high as 24.8 (95% CI 2.3-262.6) in FDR subjects with BMI ≥25 compared to the corresponding non-FDR subjects. Conclusions: High-fat diet induced insulin resistance and increase of IL-6 plasma in healthy adult Indonesian males. Immune response polarization favouring proinflammatory environment was predominantly occurred in FDR subjects when compared to those of non-FDR subjects. Alteration of lipid accumulation was highly likely contributed to greater HFD-inflammation effects on FDR than non-FDR subjects.
AB - Objectives: Non-diabetic first-degree relatives (FDR) of type 2 diabetes mellitus (T2DM) patients have been reported to have relatively higher insulin resistance and inflammatory markers compared to population without family history of T2DM. We investigated whether healthy FDR T2DM of Indonesian males living in urban area are more susceptible to the adverse effects of high-fat diet (HFD) than non-FDR subjects. Methods: Twenty-seven normoglycemic and normotensive FDR males and 28 age-and-body-mass-index-(BMI)-matched healthy non-FDR males underwent a 5-days HFD challenge. Dietary intake before and after HFD were collected by 24-hours food recall. Metabolic profiles and plasma cytokine levels were assessed before and after the HFD intervention. Results: Within similar BMI profile between groups, FDR subjects showed significantly bigger waist circumference (p=0.001) and higher triglyceride (p=0,03) than those of non-FDR. Despite similar HOMA-IR and IL-6 responses to 5-days HFD, significant increase of plasma TNF-α/IL-10 ratio found in FDR subjects, while in contrary, TNF-α/IL-10 ratio significantly decreased in non-FDR group (p<0.001), resulting an OR of 7.1 (95% CI 2.2-23.4) for FDR to develop elevated plasma TNF-α/IL-10 ratio in response to HFD. The tendency was as high as 24.8 (95% CI 2.3-262.6) in FDR subjects with BMI ≥25 compared to the corresponding non-FDR subjects. Conclusions: High-fat diet induced insulin resistance and increase of IL-6 plasma in healthy adult Indonesian males. Immune response polarization favouring proinflammatory environment was predominantly occurred in FDR subjects when compared to those of non-FDR subjects. Alteration of lipid accumulation was highly likely contributed to greater HFD-inflammation effects on FDR than non-FDR subjects.
KW - first-degree relatives
KW - high-fat diet
KW - insulin resistance
KW - TNF-a/IL-10 ratio
KW - type 2 diabetes mellitus
UR - http://www.scopus.com/inward/record.url?scp=85163868145&partnerID=8YFLogxK
U2 - 10.1900/RDS.2023.19.51
DO - 10.1900/RDS.2023.19.51
M3 - Article
AN - SCOPUS:85163868145
SN - 1613-6071
VL - 19
SP - 51
EP - 61
JO - Review of Diabetic Studies
JF - Review of Diabetic Studies
IS - 2
ER -