TY - JOUR
T1 - Mesenchymal stem cells administration in aged male rats increases testosterone and lower TNF-α levels
AU - Dwijayanti, Adisti
AU - Fitrianto, Alif Iman
AU - Boediono, Arief
AU - Setiati, Siti
AU - Suyatna, Franciscus D.
N1 - Funding Information:
Authors thank Stem Cell Institute Kalbe, Jakarta, Indonesia for preparing the human umbilical cord mesenchymal stem cells and BUDI-DN LPDP for funding this study (Contract number PRJ-5885/ LPDP.3/2016).
Publisher Copyright:
© 2021 Japan University of Health Sciences & Japan International Cultural Exchange Foundation.
PY - 2021/12
Y1 - 2021/12
N2 - Background: Treatment for sex hormone depletion along with chronic inflammation would be beneficial for aging males. Mesenchymal stem cells (MSC) are known for their immunomodulatory activities and differentiation ability in regenerative medicine. Whether MSC could influence both testosterone and pro-inflammatory marker in aging males is uncertain. This study aimed to explore the effects of human umbilical mesenchymal stem cell (hUCMSC) on testosterone, tumor necrosis factor-alpha (TNF-α), and creatinine levels in aged rats. Methods: The hUCMSC was administered to aged male Sprague-Dawley rats (24 months old). After four injections of 1 mil-lion per kg body weight in 3-month intervals, the rats were sacrificed, and serum was collected for biochemical examinations. Results: The hUCMSC administrations increased the testosterone level almost three-fold and decreased the TNF-α level. Moreover, the high testosterone level was strongly correlated with low TNF-α level (p = 0.013; r =-0.863) in aged male rats. These results were following our previous report, which showed that the hUCMSC increased the number of Leydig cells. Serum creatinine levels in the treatment group were slightly increased but were still within the normal limit. Conclusion: The hUCMSC treatment in aged male rats tends to increase testosterone levels and lower TNF-α levels.
AB - Background: Treatment for sex hormone depletion along with chronic inflammation would be beneficial for aging males. Mesenchymal stem cells (MSC) are known for their immunomodulatory activities and differentiation ability in regenerative medicine. Whether MSC could influence both testosterone and pro-inflammatory marker in aging males is uncertain. This study aimed to explore the effects of human umbilical mesenchymal stem cell (hUCMSC) on testosterone, tumor necrosis factor-alpha (TNF-α), and creatinine levels in aged rats. Methods: The hUCMSC was administered to aged male Sprague-Dawley rats (24 months old). After four injections of 1 mil-lion per kg body weight in 3-month intervals, the rats were sacrificed, and serum was collected for biochemical examinations. Results: The hUCMSC administrations increased the testosterone level almost three-fold and decreased the TNF-α level. Moreover, the high testosterone level was strongly correlated with low TNF-α level (p = 0.013; r =-0.863) in aged male rats. These results were following our previous report, which showed that the hUCMSC increased the number of Leydig cells. Serum creatinine levels in the treatment group were slightly increased but were still within the normal limit. Conclusion: The hUCMSC treatment in aged male rats tends to increase testosterone levels and lower TNF-α levels.
KW - Aging
KW - Mesenchymal stem cell
KW - Testosterone
KW - TNF-α
UR - http://www.scopus.com/inward/record.url?scp=85121461058&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85121461058
SN - 1341-2051
VL - 28
SP - 601
EP - 603
JO - International Medical Journal
JF - International Medical Journal
IS - 6
ER -
