Mesenchymal stem cells administration in aged male rats increases testosterone and lower TNF-α levels

Adisti Dwijayanti, Alif Iman Fitrianto, Arief Boediono, Siti Setiati, Franciscus D. Suyatna

Research output: Contribution to journalArticlepeer-review


Background: Treatment for sex hormone depletion along with chronic inflammation would be beneficial for aging males. Mesenchymal stem cells (MSC) are known for their immunomodulatory activities and differentiation ability in regenerative medicine. Whether MSC could influence both testosterone and pro-inflammatory marker in aging males is uncertain. This study aimed to explore the effects of human umbilical mesenchymal stem cell (hUCMSC) on testosterone, tumor necrosis factor-alpha (TNF-α), and creatinine levels in aged rats. Methods: The hUCMSC was administered to aged male Sprague-Dawley rats (24 months old). After four injections of 1 mil-lion per kg body weight in 3-month intervals, the rats were sacrificed, and serum was collected for biochemical examinations. Results: The hUCMSC administrations increased the testosterone level almost three-fold and decreased the TNF-α level. Moreover, the high testosterone level was strongly correlated with low TNF-α level (p = 0.013; r =-0.863) in aged male rats. These results were following our previous report, which showed that the hUCMSC increased the number of Leydig cells. Serum creatinine levels in the treatment group were slightly increased but were still within the normal limit. Conclusion: The hUCMSC treatment in aged male rats tends to increase testosterone levels and lower TNF-α levels.

Original languageEnglish
Pages (from-to)601-603
Number of pages3
JournalInternational Medical Journal
Issue number6
Publication statusPublished - Dec 2021


  • Aging
  • Mesenchymal stem cell
  • Testosterone
  • TNF-α


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