TY - JOUR
T1 - Mesenchymal stem cell aging, their environment and methods to restore their quality
AU - Dwijayanti, Adisti
AU - Pawitan, Jeanne Adiwinata
N1 - Funding Information:
This work was supported by a research grant from the Ministry of Research, Technology and Higher Education of the Republic of Indonesia, Hibah Penelitian Pengembangan 2019, contract no.NKB-1804/UN2.R3.1/HKP.05.00/2019
Publisher Copyright:
©2021 Japan University of Health Sciences & Japan International Cultural Exchange Foundation.
PY - 2021/10
Y1 - 2021/10
N2 - Objective: This article aims to explore the aging of mesenchymal stem cells (MSCs) during cell expansion and available methods to restore their quality. Materials and methods: we searched articles in PubMed/Medline and our library to collect relevant publications of MSC aging, their microenvironment (niche), and various approaches to prevent MSC aging. Results and Discussion: MSC expansion during culture may cause cell aging, which is reflected by change in growth, geno-type, and phenotype that can be seen in morphology and function. There are various approaches that can be used to prevent MSC aging. Approaches to prevent MSC aging in culture can be done by various methods, including by determining donor characteristics, genetic engineering approaches, regulating stem cells microenvironment in vitro, and in vitro physical modification Conclusion: Prevention of MSC aging during culture can be done by selection of MSC sources, genetic modification, and microenvironment and physical adjustments.
AB - Objective: This article aims to explore the aging of mesenchymal stem cells (MSCs) during cell expansion and available methods to restore their quality. Materials and methods: we searched articles in PubMed/Medline and our library to collect relevant publications of MSC aging, their microenvironment (niche), and various approaches to prevent MSC aging. Results and Discussion: MSC expansion during culture may cause cell aging, which is reflected by change in growth, geno-type, and phenotype that can be seen in morphology and function. There are various approaches that can be used to prevent MSC aging. Approaches to prevent MSC aging in culture can be done by various methods, including by determining donor characteristics, genetic engineering approaches, regulating stem cells microenvironment in vitro, and in vitro physical modification Conclusion: Prevention of MSC aging during culture can be done by selection of MSC sources, genetic modification, and microenvironment and physical adjustments.
KW - Aging
KW - Cell culture
KW - Mesenchymal stem cells
KW - Micro environment
KW - Propagation
UR - http://www.scopus.com/inward/record.url?scp=85117117062&partnerID=8YFLogxK
M3 - Article
AN - SCOPUS:85117117062
VL - 28
SP - 520
EP - 523
JO - International Medical Journal
JF - International Medical Journal
SN - 1341-2051
IS - 5
ER -