Currently, many organizations have adopted agile software development (ASD) methods. Although ASD is widely known as a better way of developing software, its implementation also has challenges that need special attention. Therefore, some research revealed various dimensions of factors that can potentially maximize the implementation success of ASD methods. Still, none of them specifically examined the dimensions of organizational culture, even though the previous survey and several studies have discovered it as an essential factor determining the ASD Project Success. Consequently, this research tries to identify how the organizational culture affects the success of ASD projects. This study aims to provide a new research model regarding organizational culture and project success in ASD and its validation. The validation process uses the Partial Least Squares-Structural Equation Modeling (PLS-SEM) technique. The findings show that Rational Culture and Group Culture form positively impacts ASD Project Success. Meanwhile, Hierarchical Culture form negatively impacts the ASD Project Success. Particularly for Development Culture, there is a positive impact on the ASD Project Success only if there is mediation from the ASD Practices.