Mass rapid transit (MRT) is an efficient transportation mode that is urgently needed by a growing city such as Jakarta, Indonesia. However, limited research has attempted to evaluate the system's current performance through a comprehensive, unit-based calculation of the costs of MRT operation and maintenance. This research aimed to develop a system for calculating and comparing MRT operation and maintenance costs per kilometer per year. The cost model has three components, namely, capital, operation cost, and maintenance cost, which are, respectively, calculated based on their percentage toward total cost. The cost model calculation determined that Jakarta MRT operation and maintenance costs total USD 8.44 million per kilometer per year. This result was compared to other countries' MRT operations.