Marker of lipid peroxidation related to diabetic nephropathy in Indonesian type 2 diabetes mellitus patients

Rani Sauriasari, Retnosari Andrajati, Azizahwati, Dharmeizar, D. A. Saputri, R. U. Muris, A. Manfaatun, O. F. Amanda, H. Setiawan, N. Sakano, D. H. Wang, K. Ogino

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Objective: Even though diabetes patients exhibit an increased oxidative stress, its correlation with diabetic nephropathy is not fully understood. The purpose of this study was to determine whether lipid peroxidation marker correlates well with eGFR and UACR in type 2 diabetes mellitus patients. Methods: We collected urine and serum samples of Indonesian type 2 diabetes mellitus outpatients with normo- and microalbuminuria at a Local Government Clinic (from ages: 39-74 years). Urinary 8-iso-PGF was measured by ELISA, the serum malondialdehyde by TBARS assay, and urinary albumin by BCG albumin assay. eGFR was calculated using the corrected-Cockcroft-Gault (CG), MDRD, and CKD-EPI equation. Other necessary data were obtained through questionnaires. Results: The results showed that the increasing level of malondialdehyde was mildly correlated with the decline in eGFR (MDRD). In contrary, there was a significant positive correlation between 8-iso-PGF concentration and eGFR based on the corrected-CG, MDRD study, and CKD-EPI equation (r=0.457, p<0.001; r=0.424, p<0.001; r=0.443, p<0.001). This relationship still persisted in the normoalbuminuric subjects (n=43) (r=0.491, p=0.001; r=0.461, p=0.002; r=0.455, p=0.002). The multivariate analysis showed that 8-iso-PGF together with fasting plasma glucose was the most predictive factor for the high 2-quantile eGFR (adjusted OR 1.001, (95% CI, 1.000-1.001)). However, there was no significant correlation between UACR with malondialdehyde (r=0.268, p=0.050) and 8-iso-PGF(r=-0.030, p=0.808). UACR itself was inversely correlated with eGFR based on the corrected-CG, the MDRD, and CKD-EPI (r=-0.232, p<0.05; r=-0.228, p<0.05; r=-0.232, p<0.05). Conclusions: Increased 8-iso-PGF and malondialdehyde in type 2 diabetes mellitus patients may play a role in the pathophysiologic significance of diabetic nephropathy, even while considering the effect of potential confounders.

Original languageEnglish
Pages (from-to)193-200
Number of pages8
JournalDiabetes Research and Clinical Practice
Volume108
Issue number1
DOIs
Publication statusPublished - 1 Apr 2015

Keywords

  • 8-iso-PGF
  • Diabetes mellitus
  • Estimated glomerular filtration rate
  • Urine albumin to creatinine ratio

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