Manufacturing and Testing the Antibacterial Activity of Cytotoxicity by Lethality Test of Brine Shrimp, Antibacterial Activity of Ripe Fruit Flesh Mangifera quadrifida Jack

Isem kembang (Mangifera quadrifida Jack) is part of the Mangifera clan of the Anacardiaceae tribe. Research on the benefits of ripe Mangifera quadrifida Jack fruit as an antibacterial (antimicrobial) and cytotoxic agent with Brine Shrimp of Lethality Test (BSLT) have been successfully conducted.This research was conducted in two stages, namely the extraction of the ripe fruit flesh of Mangifera quadrifida Jack with the maceration method using n-hexane, ethyl acetate, and methanol, followed by antibacterial testing using the well-diffusion method (disk diffusion assay) and cytotoxicity testing using the Lethality Test of Brine Shrimp. According to the results the extraction of ripe fruit flesh Mangifera quadrifida Jack shows that ethyl acetate is the best solvent for extracting and killing bacteria, the methanol extract of ripe fruit flesh from Mangifera quadrifida Jack has potential as a compound antitumor or anticancer drug as the Brine Shrimp Lethality Test (BSLT) method show acute toxicity to Artemia salina Leach larvae.