Magnetic enzymatic platform for organophosphate pesticide detection using boron-doped diamond electrodes

Flavio Pino, Ivandini Tribidasari Anggraningrum, Kazuya Nakata, Akira Fujishima, Arben Merkoçi, Yasuaki Einaga

Research output: Contribution to journalArticlepeer-review

14 Citations (Scopus)


A simple and reliable enzymatic system for organophosporus pesticide detection was successfully developed, by exploiting the synergy between the magnetic beads collection capacity and the outstanding electrochemistry property of boron-doped diamond electrodes. The determination of an organophosphate pesticide, chlorpyrifos (CPF), was performed based on the inhibition system of the enzyme acetylcholinesterase bonded to magnetic beads through a biotin-streptavidin complex system. A better sensitivity was found for a system with magnetic beads in the concentration range of 10 -9 to 10 -5 M. The estimated limits of detection based on IC 10 (10% acetylcholinesterase (AChE) inhibition) have been detected and optimized to be 5.7 × 10 -10 M CPF. Spiked samples of water of Yokohama (Japan) have been measured to validate the efficiency of the enzymatic system. The results suggested that the use of magnetic beads to immobilize biomolecules or biosensing agents is suitable to maintain the superiority of BDD electrodes.

Original languageEnglish
Pages (from-to)1061-1068
Number of pages8
JournalAnalytical Sciences
Issue number10
Publication statusPublished - 2015


  • Boron-doped diamond electrode
  • Inhibition based detection system
  • Magnetic pre-concentration platform
  • Organophosphate


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