Magnetic enzymatic platform for organophosphate pesticide detection using boron-doped diamond electrodes

Flavio Pino; Ivandini Tribidasari Anggraningrum; Kazuya Nakata; Akira Fujishima; Arben Merkoçi; Yasuaki Einaga

doi:10.2116/analsci.31.1061

Magnetic enzymatic platform for organophosphate pesticide detection using boron-doped diamond electrodes

Flavio Pino, Ivandini Tribidasari Anggraningrum, Kazuya Nakata, Akira Fujishima, Arben Merkoçi, Yasuaki Einaga

Department of Chemistry

Research output: Contribution to journal › Article › peer-review

15 Citations (Scopus)

Abstract

A simple and reliable enzymatic system for organophosporus pesticide detection was successfully developed, by exploiting the synergy between the magnetic beads collection capacity and the outstanding electrochemistry property of boron-doped diamond electrodes. The determination of an organophosphate pesticide, chlorpyrifos (CPF), was performed based on the inhibition system of the enzyme acetylcholinesterase bonded to magnetic beads through a biotin-streptavidin complex system. A better sensitivity was found for a system with magnetic beads in the concentration range of 10 ^-9 to 10 ^-5 M. The estimated limits of detection based on IC ₁₀ (10% acetylcholinesterase (AChE) inhibition) have been detected and optimized to be 5.7 × 10 ^-10 M CPF. Spiked samples of water of Yokohama (Japan) have been measured to validate the efficiency of the enzymatic system. The results suggested that the use of magnetic beads to immobilize biomolecules or biosensing agents is suitable to maintain the superiority of BDD electrodes.

Original language	English
Pages (from-to)	1061-1068
Number of pages	8
Journal	Analytical Sciences
Volume	31
Issue number	10
DOIs	https://doi.org/10.2116/analsci.31.1061
Publication status	Published - 2015

Keywords

Boron-doped diamond electrode
Inhibition based detection system
Magnetic pre-concentration platform
Organophosphate

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10.2116/analsci.31.1061

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title = "Magnetic enzymatic platform for organophosphate pesticide detection using boron-doped diamond electrodes",

abstract = " A simple and reliable enzymatic system for organophosporus pesticide detection was successfully developed, by exploiting the synergy between the magnetic beads collection capacity and the outstanding electrochemistry property of boron-doped diamond electrodes. The determination of an organophosphate pesticide, chlorpyrifos (CPF), was performed based on the inhibition system of the enzyme acetylcholinesterase bonded to magnetic beads through a biotin-streptavidin complex system. A better sensitivity was found for a system with magnetic beads in the concentration range of 10 -9 to 10 -5 M. The estimated limits of detection based on IC 10 (10% acetylcholinesterase (AChE) inhibition) have been detected and optimized to be 5.7 × 10 -10 M CPF. Spiked samples of water of Yokohama (Japan) have been measured to validate the efficiency of the enzymatic system. The results suggested that the use of magnetic beads to immobilize biomolecules or biosensing agents is suitable to maintain the superiority of BDD electrodes.",

keywords = "Boron-doped diamond electrode, Inhibition based detection system, Magnetic pre-concentration platform, Organophosphate",

author = "Flavio Pino and Anggraningrum, {Ivandini Tribidasari} and Kazuya Nakata and Akira Fujishima and Arben Merko{\c c}i and Yasuaki Einaga",

note = "Publisher Copyright: {\textcopyright} 2015 The Japan Society for Analytical Chemistry.",

year = "2015",

doi = "10.2116/analsci.31.1061",

language = "English",

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T1 - Magnetic enzymatic platform for organophosphate pesticide detection using boron-doped diamond electrodes

AU - Pino, Flavio

AU - Anggraningrum, Ivandini Tribidasari

AU - Nakata, Kazuya

AU - Fujishima, Akira

AU - Merkoçi, Arben

AU - Einaga, Yasuaki

PY - 2015

Y1 - 2015

N2 - A simple and reliable enzymatic system for organophosporus pesticide detection was successfully developed, by exploiting the synergy between the magnetic beads collection capacity and the outstanding electrochemistry property of boron-doped diamond electrodes. The determination of an organophosphate pesticide, chlorpyrifos (CPF), was performed based on the inhibition system of the enzyme acetylcholinesterase bonded to magnetic beads through a biotin-streptavidin complex system. A better sensitivity was found for a system with magnetic beads in the concentration range of 10 -9 to 10 -5 M. The estimated limits of detection based on IC 10 (10% acetylcholinesterase (AChE) inhibition) have been detected and optimized to be 5.7 × 10 -10 M CPF. Spiked samples of water of Yokohama (Japan) have been measured to validate the efficiency of the enzymatic system. The results suggested that the use of magnetic beads to immobilize biomolecules or biosensing agents is suitable to maintain the superiority of BDD electrodes.

AB - A simple and reliable enzymatic system for organophosporus pesticide detection was successfully developed, by exploiting the synergy between the magnetic beads collection capacity and the outstanding electrochemistry property of boron-doped diamond electrodes. The determination of an organophosphate pesticide, chlorpyrifos (CPF), was performed based on the inhibition system of the enzyme acetylcholinesterase bonded to magnetic beads through a biotin-streptavidin complex system. A better sensitivity was found for a system with magnetic beads in the concentration range of 10 -9 to 10 -5 M. The estimated limits of detection based on IC 10 (10% acetylcholinesterase (AChE) inhibition) have been detected and optimized to be 5.7 × 10 -10 M CPF. Spiked samples of water of Yokohama (Japan) have been measured to validate the efficiency of the enzymatic system. The results suggested that the use of magnetic beads to immobilize biomolecules or biosensing agents is suitable to maintain the superiority of BDD electrodes.

KW - Boron-doped diamond electrode

KW - Inhibition based detection system

KW - Magnetic pre-concentration platform

KW - Organophosphate

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Magnetic enzymatic platform for organophosphate pesticide detection using boron-doped diamond electrodes

Abstract

Keywords

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