TY - JOUR
T1 - Lymphokine activated killer cells from peripheral blood mononuclear cells of endometriosis of patients improve cytotoxicity to endometriosis cell culture
AU - Natadisastra, Muharam
AU - Arleni, null
AU - Mansur, Indra Gusti
AU - Jacoeb, Teuku Zulkifli
AU - Giustiniani, Jerome
AU - Schiavon, Valerie
AU - Bensussan, Armand
N1 - Funding Information:
We thank the staffs of INSERM U976 Saint Louis Hospital-Paris VII, for their support in conducting this research. We also thank MTIE FMUI and Division of Reproductive Immunoendocrinology FMUI, especially to Professors Endy M. Moegni, Ali Baziad, Soetarti Eko Prasetyaningsih, Eva, Nilda, and Neneng.
Publisher Copyright:
© 2011, Faculty of Medicine, Universitas Indonesia. All rights reserved.
PY - 2011/5
Y1 - 2011/5
N2 - Background: To assess the increased cellular immunity of Peripheral Blood Mononuclear Cells (PBMC) derived LAK cells from endometriosis patients towards endometriosis cell cultures after stimulation with IL-2. Methods: This study is a quasi-experimental study of pre and post treatment using controls. Phenotype evaluation of CD3+CD4+, CD3+CD8+ and CD56+ effector cells of PBMC from endometriosis patients and controls was performed. Cytotoxicity test of PBMC from endometriosis patients and control towards Daudi, K562 cell line and endometriosis cell cultures using 51Chromium release assay was also carried out. Results: Phenotype evaluation of PBMC from endometriosis patients (n=10) and controls (n=6) were done prior to and after IL-2 stimulation. Before IL-2 stimulation, CD3+CD4+, CD56+ from endometriosis group (n=10) tend to be lower than control (n=6) whereas CD3+CD8 + were higher in endometriosis group than controls. After IL-2 stimulation, CD3+ CD8+, CD56+ of PBMC from endometriosis group were significantly increased (p <0.05).Cytotoxicity test revealed a significant increase (p <0.05) in both PBMC’s effector cells from endometriosis and control group towards target cells, Daudi, and K562 cell lines after IL-2 stimulation. PBMC’s effector cells cytotoxicity from both endometriosis and control towards target endometriosis cell cultures were also elevated after IL-2 stimulation. Conclusion: LAK cells derived IL-2 stimulated PBMC from endometriosis patients increased cellular immunity towards endometriosis cell cultures.
AB - Background: To assess the increased cellular immunity of Peripheral Blood Mononuclear Cells (PBMC) derived LAK cells from endometriosis patients towards endometriosis cell cultures after stimulation with IL-2. Methods: This study is a quasi-experimental study of pre and post treatment using controls. Phenotype evaluation of CD3+CD4+, CD3+CD8+ and CD56+ effector cells of PBMC from endometriosis patients and controls was performed. Cytotoxicity test of PBMC from endometriosis patients and control towards Daudi, K562 cell line and endometriosis cell cultures using 51Chromium release assay was also carried out. Results: Phenotype evaluation of PBMC from endometriosis patients (n=10) and controls (n=6) were done prior to and after IL-2 stimulation. Before IL-2 stimulation, CD3+CD4+, CD56+ from endometriosis group (n=10) tend to be lower than control (n=6) whereas CD3+CD8 + were higher in endometriosis group than controls. After IL-2 stimulation, CD3+ CD8+, CD56+ of PBMC from endometriosis group were significantly increased (p <0.05).Cytotoxicity test revealed a significant increase (p <0.05) in both PBMC’s effector cells from endometriosis and control group towards target cells, Daudi, and K562 cell lines after IL-2 stimulation. PBMC’s effector cells cytotoxicity from both endometriosis and control towards target endometriosis cell cultures were also elevated after IL-2 stimulation. Conclusion: LAK cells derived IL-2 stimulated PBMC from endometriosis patients increased cellular immunity towards endometriosis cell cultures.
KW - Daudi cell line
KW - Endometriosis
KW - Endometriosis cell cultures
KW - K562 cell line
KW - LAK cells
KW - NK cells
UR - http://www.scopus.com/inward/record.url?scp=85008698043&partnerID=8YFLogxK
U2 - 10.13181/mji.v20i2.434
DO - 10.13181/mji.v20i2.434
M3 - Article
AN - SCOPUS:85008698043
SN - 0853-1773
VL - 20
SP - 87
EP - 93
JO - Medical Journal of Indonesia
JF - Medical Journal of Indonesia
IS - 2
ER -