TY - JOUR
T1 - Liquid chromatography-tandem mass spectrometric analyses of the amino acid profiles of normal and malnourished children treated at Cipto Mangunkusumo Hospital
AU - Kosen, D. N.
AU - Timan, I. S.
AU - Sjarif, D. R.
N1 - Publisher Copyright:
© 2018 Institute of Physics Publishing. All rights reserved.
PY - 2018/9/7
Y1 - 2018/9/7
N2 - Children require dietary protein for growth. The nutritional quality of proteins is influenced by their amino acid composition. Amino acids are required for life, contributing to homeostasis, immunity, growth, and development. In this preliminary study, we used liquid chromatography-tandem mass spectrometry (LC-MS/MS) to determine the amino acid profiles of glycine, alanine, proline, valine, leucine, ornithine, methionine, phenylalanine, arginine, citrulline, tyrosine, aspartic acid, and glutamic acid of normal and malnourished children treated at Cipto Mangunkusumo Hospital. A total of 60 subjects were analyzed from December 2016 to April 2017. Dry blood spots were analyzed using LC-MS/MS. The study subjects comprised 12 malnourished and 18 normal children. The mean weight, height, and z-score (weight-for-age, height-for-age, and weight-for-height) were significantly lower in the malnourished group. The coefficients of variation percentage among runs ranged from 1.76% to 12.03%. There was no significant difference between the amino acid profiles of the normal and malnourished groups, although the concentrations of glycine and glutamate were significantly higher in the malnourished group.
AB - Children require dietary protein for growth. The nutritional quality of proteins is influenced by their amino acid composition. Amino acids are required for life, contributing to homeostasis, immunity, growth, and development. In this preliminary study, we used liquid chromatography-tandem mass spectrometry (LC-MS/MS) to determine the amino acid profiles of glycine, alanine, proline, valine, leucine, ornithine, methionine, phenylalanine, arginine, citrulline, tyrosine, aspartic acid, and glutamic acid of normal and malnourished children treated at Cipto Mangunkusumo Hospital. A total of 60 subjects were analyzed from December 2016 to April 2017. Dry blood spots were analyzed using LC-MS/MS. The study subjects comprised 12 malnourished and 18 normal children. The mean weight, height, and z-score (weight-for-age, height-for-age, and weight-for-height) were significantly lower in the malnourished group. The coefficients of variation percentage among runs ranged from 1.76% to 12.03%. There was no significant difference between the amino acid profiles of the normal and malnourished groups, although the concentrations of glycine and glutamate were significantly higher in the malnourished group.
UR - http://www.scopus.com/inward/record.url?scp=85054497575&partnerID=8YFLogxK
U2 - 10.1088/1742-6596/1073/3/032045
DO - 10.1088/1742-6596/1073/3/032045
M3 - Conference article
AN - SCOPUS:85054497575
SN - 1742-6588
VL - 1073
JO - Journal of Physics: Conference Series
JF - Journal of Physics: Conference Series
IS - 3
M1 - 032045
T2 - 2nd Physics and Technologies in Medicine and Dentistry Symposium, PTMDS 2018
Y2 - 18 July 2018 through 18 July 2018
ER -