Lipase production from Bacillus subtilis with submerged fermentation using waste cooking oil

Bacterial lipase has been developed lately because of its advantage to produce with large scale. Culture of Bacillus subtilis was grown to produce lipase in Waste Cooking Oil (WCO) using submerged fermentation (SmF) method. The enzyme activity of the culture was improved by using different concentration of inoculum, substrate, nitrogen source, inducer, and Ca²⁺ ion at 30°C for 84h fermentation. Lipolytic activity of crude lipase was determined using titrimetric method with hydrolysis reaction. Maximum activity of lipase (4.96 U/mL) was found at 5% (v/v) inoculum, 4% (v/v) WCO, 0.5% (w/v) yeast extract, 0.25% (v/v) olive oil, and 10 mM Ca²⁺ that present in medium culture. Later, the crude lipase has been dried with spray dryer and resulting 17.33 gr of dry lipase powder per 500 mL crude lipase.