TY - GEN
T1 - Lipase biocatalyst immobilization from solid state fermentation of palm oil empty fruit bunches, bagasse, and palm oil sludge with adsorption-cross linking method in anion macroporous resin
AU - Hartono, Rudi
AU - Hannauli, Yessica S.
AU - Maresya, Ambar
AU - Sahlan, Muhamad
AU - Wijanarko, Anondho
AU - Gozan, Misri
AU - Meyer, Michel
AU - Hermansyah, Heri
N1 - Publisher Copyright:
© 2018 Trans Tech Publications, Switzerland.
PY - 2018
Y1 - 2018
N2 - Enzymes are used to make a production of biomass based process more efficient and more selective. Lipase is a biocatalyst in the breakdown of fat reaction that is widely used in the industry, including the industry in Indonesia. Lipase production can be carried out by bacterial solid state fermentation by using agro-industry waste substrate containing carbon and nitrogen. The developments of various industrial sectors are demanding the use and application of lipase commercially in industrial processes as biocatalysts chosen by its ability to work in a friendly environment and have high specificity. Fermentation of Aspergillus niger are able to produce enzyme lipase that can be done by using solid-state fermentation method. In this study palm oil empty fruit bunches (POEFB), bagasse, and palm oil sludge are used as fermentation substrates and will be treated variations of inducer concentration and fermentation time. The results of solid state fermentation of solid substrates POEFB with inducer concentration of 8% for 7 days showed the highest activity value of 2.2 U/mL and 8.2 U/mL in the form of dry extract lipase. The result of the dry lipase enzyme immobilized so that enzyme was stable in repetitive use with cross linking adsorption method using macroporous resin as a support. Experiments showed that empty fruit bunches of oil palm fermentation substrate could produce lipase enzyme with enzyme loading of 56.6% wt. Enzyme activity test carried out in the synthesis of biodiesel through interesterification reaction mole ratio of reactants palm oil and metal acetate 1:12 at 40o C operating temperature conditions for 50 hours in 4 reaction cycles. Biodiesel synthesis results were analyzed using High Perfomance Liquid Chromatography (HPLC) showed biodiesel yield values of 48.6% and the enzyme was able to move up to 68.60% initial yield of 4 cycles of biodiesel synthesis.
AB - Enzymes are used to make a production of biomass based process more efficient and more selective. Lipase is a biocatalyst in the breakdown of fat reaction that is widely used in the industry, including the industry in Indonesia. Lipase production can be carried out by bacterial solid state fermentation by using agro-industry waste substrate containing carbon and nitrogen. The developments of various industrial sectors are demanding the use and application of lipase commercially in industrial processes as biocatalysts chosen by its ability to work in a friendly environment and have high specificity. Fermentation of Aspergillus niger are able to produce enzyme lipase that can be done by using solid-state fermentation method. In this study palm oil empty fruit bunches (POEFB), bagasse, and palm oil sludge are used as fermentation substrates and will be treated variations of inducer concentration and fermentation time. The results of solid state fermentation of solid substrates POEFB with inducer concentration of 8% for 7 days showed the highest activity value of 2.2 U/mL and 8.2 U/mL in the form of dry extract lipase. The result of the dry lipase enzyme immobilized so that enzyme was stable in repetitive use with cross linking adsorption method using macroporous resin as a support. Experiments showed that empty fruit bunches of oil palm fermentation substrate could produce lipase enzyme with enzyme loading of 56.6% wt. Enzyme activity test carried out in the synthesis of biodiesel through interesterification reaction mole ratio of reactants palm oil and metal acetate 1:12 at 40o C operating temperature conditions for 50 hours in 4 reaction cycles. Biodiesel synthesis results were analyzed using High Perfomance Liquid Chromatography (HPLC) showed biodiesel yield values of 48.6% and the enzyme was able to move up to 68.60% initial yield of 4 cycles of biodiesel synthesis.
KW - Adsorption
KW - Agro-industrial waste
KW - Cross Linking
KW - Lipase Immobilization
UR - http://www.scopus.com/inward/record.url?scp=85055491758&partnerID=8YFLogxK
U2 - 10.4028/www.scientific.net/MSF.929.209
DO - 10.4028/www.scientific.net/MSF.929.209
M3 - Conference contribution
AN - SCOPUS:85055491758
SN - 9783035712667
T3 - Materials Science Forum
SP - 209
EP - 217
BT - Advanced Materials Research - QiR 15
A2 - Yatim, Ardiyansyah
PB - Trans Tech Publications Ltd
T2 - 15th International Conference on Quality in Research, QiR 2017
Y2 - 24 July 2017 through 27 July 2017
ER -