TY - JOUR
T1 - Lignocellulolytic enzyme activity pattern of three white oyster mushroom (Pleurotus ostreatus (Jacq.) P. Kumm.) strains during mycelial growth and fruiting body development
AU - Nurfitri, N.
AU - Mangunwardoyo, W.
AU - Saskiawan, I.
N1 - Funding Information:
We would like to extend our gratitude to Dr. Iwan Saskiawan who served as major contributor and to Laboratory of Food Microbiology, Research Center for Biology, Indonesian Institute of Sciences (LIPI) for funding and facilitating this research.
Publisher Copyright:
© 2021 Journal of Physics: Conference Series.
Copyright:
Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2021/1/12
Y1 - 2021/1/12
N2 - Pleurotus ostreatus is one of the edible mushrooms that can utilize lignocellulose as substrate because of their ability to secrete the lignocellulolytic enzyme. The purpose of this research is to investigate and compare the lignocellulolytic enzymes production of P. ostreatus InaCC F209, F216 and LIPI on solid-state fermentation using sawdust during 70 days of mycelial growth (vegetative phase) and fruiting body development (reproductive phase). Supernatant of the extracted enzyme solutions were employed to investigate the reducing sugar, soluble protein, and enzyme activities. The results revealed that reducing sugar concentration of the three P. ostreatus strains subjected increased during growth and reached the maximum concentration on the reproductive phase, while the total protein content fluctuated during the growth but reached the maximum concentration on the reproductive phase. Laccase, LiP, and MnP activities of three P. ostreatus strains were higher on the vegetative phase, while the endoxylanase and endoglucanase activities were higher on the reproductive phase. β-glucosidase activity showed different variations between three P. ostreatus strains. Pleurotus ostreatus InaCC F209 produced the highest and most stable laccase, β-glucosidase, endoglucanase, and endoxylanase than two others.
AB - Pleurotus ostreatus is one of the edible mushrooms that can utilize lignocellulose as substrate because of their ability to secrete the lignocellulolytic enzyme. The purpose of this research is to investigate and compare the lignocellulolytic enzymes production of P. ostreatus InaCC F209, F216 and LIPI on solid-state fermentation using sawdust during 70 days of mycelial growth (vegetative phase) and fruiting body development (reproductive phase). Supernatant of the extracted enzyme solutions were employed to investigate the reducing sugar, soluble protein, and enzyme activities. The results revealed that reducing sugar concentration of the three P. ostreatus strains subjected increased during growth and reached the maximum concentration on the reproductive phase, while the total protein content fluctuated during the growth but reached the maximum concentration on the reproductive phase. Laccase, LiP, and MnP activities of three P. ostreatus strains were higher on the vegetative phase, while the endoxylanase and endoglucanase activities were higher on the reproductive phase. β-glucosidase activity showed different variations between three P. ostreatus strains. Pleurotus ostreatus InaCC F209 produced the highest and most stable laccase, β-glucosidase, endoglucanase, and endoxylanase than two others.
KW - Lignocellulolytic enzymes
KW - Pleurotus ostreatus InaCC F209
KW - Reproductive phase
KW - Vegetative phase
UR - http://www.scopus.com/inward/record.url?scp=85100786720&partnerID=8YFLogxK
U2 - 10.1088/1742-6596/1725/1/012056
DO - 10.1088/1742-6596/1725/1/012056
M3 - Conference article
AN - SCOPUS:85100786720
SN - 1742-6588
VL - 1725
JO - Journal of Physics: Conference Series
JF - Journal of Physics: Conference Series
IS - 1
M1 - 012056
T2 - 2nd Basic and Applied Sciences Interdisciplinary Conference 2018, BASIC 2018
Y2 - 3 August 2018 through 4 August 2018
ER -