LIF and LIF-R protein expression in macaca nemestrina midluteal endometrial tissues after controlled ovarium stimulation

Implantation rate in In Vitro Fertilization (IVF) as assisted reproductive technology has been remained low and suspected being caused by the negative effect of 7controlled ovarium stimulation (COS) procedure. Several studies reported that the negative effect made the maturity of endometrium patient, one of the factors was seen by biomolecular changes, with IVF occurs faster than normal. In this study, leukemia inhibitory factor (LIF) and the receptor determined in tissue endometrial of M. nemestrina to analyze the expression of them after COS. M. nemestrina stimulated with Gonadotropin Releasing Hormone (GnRH) and recombinant follicle stimulating hormone (r-FSH) using agonist protocol. There were two groups of r-FSH (4 samples in 30IU dose group and 4 samples in 50IU dose group) that used and compared the effect with 3 samples as the control group. A concentration of estrogen (E2) and progesterone (P4) from the late-follicular phase also determined to see the correlation with them. LIF and LIF-R concentration determined by ELISA assay and localization of expression determined by IHC. Statistic analyzed by One-way ANOVA test for normal distribution and Kruskal Wallis for not-normal distribution to compare LIF and LIF-R within dose group. Spearman test used to analyze the correlation of estrogen and progesterone with LIF and LIF-R. We found no statistically significant of LIF and LIF-R within group. Only correlation of progesterone and LIF-R that have statistically significant which the correlation was strong in a negative direction. Expression of LIF found in cytoplasmic and that was statistically significant within group. The expression in r-FSH groups were lower than a control group. Expression of dose LIF-R found in cytoplasmic and there was no statistically significant within dose group.