Leptin gene (Lep) G-2548A polymorphism in patients with and without osteoporosis

Leptin plays several important roles in the body, such as body mass control and bone remodeling. Previous research suggests that LEP G-2548A gene polymorphism may be associated with increased risk of osteoporosis. This study aimed at analyzing LEP G-2548A polymorphism in Indonesian patients with and without osteoporosis. DNA samples were collected from 50 subjects with osteoporosis and 50 subjects without osteoporosis. The DNA fragments were amplified with LEP G-2548A primers using polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP) analysis using the HHAI restriction enzyme. The distribution of the genotypes and alleles in the test and control groups were determined and were compared. The frequency of the AA genotype of LEP G-2548A was higher in the osteoporosis group than in the control group. However, the differences in genotype and allele frequencies between the two groups were not statistically significant (p > 0.05). The genotypes and alleles of LEP G-2548A gene polymorphism did not differ significantly between Indonesian patients with and without osteoporosis.