Latent membrane protein-1 of Epstein-Barr virus induces the expression of B-cell integration cluster, a precursor form of microRNA-155, in B lymphoma cell lines

Nur Rahadiani, Tetsuya Takakuwa, Kristianti Tresnasari, Eiichi Morii, Katsuyuki Aozasa

Research output: Contribution to journalArticlepeer-review

50 Citations (Scopus)

Abstract

miR-155, a microRNA, and its precursor form, B-cell integration cluster (BIC), are involved in tumor growth. Epstein-Barr virus (EBV)-associated malignancies are categorized into three types, based on their latent gene expression pattern: latency I, II, and III. In the present study, we found that infection with EBV increased the expression of BIC; in addition, substantial expression of BIC/miR-155 was detected in latency III-, but not in latency I-type cells. In comparison, latent membrane protein-1 (LMP1) was expressed in latency III-type cells. When LMP1 was over-expressed, BIC expression increased, indicating LMP1 mediates BIC expression. LMP1 is a membrane-associated protein known to activate signaling pathways. With the use of pathway inhibitors, we found that LMP1-induced strong BIC expression, primarily through NF-κB and p38/MAPK pathways. These results suggest that BIC/miR-155 play a role in lymphomagenesis through NF-κB and p38/MAPK pathways in response to activation by EBV LMP1.

Original languageEnglish
Pages (from-to)579-583
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume377
Issue number2
DOIs
Publication statusPublished - 12 Dec 2008

Keywords

  • BIC/miR-155
  • EB virus
  • LMP1
  • Lymphoma

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