L-DOPA synthesis using tyrosinase-immobilized on electrode surfaces

Siti Fauziyah Rahman, Siramulu Gobikhrisnan, Misri, Gwi Taek Jong, Don Hee Park

Research output: Contribution to journalArticlepeer-review

1 Citation (Scopus)

Abstract

Levodopa or L-3,4-dihydroxyphenylalanine (L-DOPA) is the direct precursor of the neurotransmitter dopamine. L-DOPA is a well-known neuroprotective agent for the treatment of Parkinson's disease symptoms. L-DOPA was synthesized using the enzyme, tyrosinase, as a biocatalyst for the conversion of L-tyrosine to L-DOPA and an electrochemical method for reducing L-DOPAquinone, the product resulting from enzymatic synthesis, to L-DOPA. In this study, three electrode systems were used: A glassy carbon electrode (GCE) as working electrode, a platinum, and a Ag/AgCl electrode as auxiliary and reference electrodes, respectively. GCE has been modified using electropolymerization of pyrrole to facilitate the electron transfer process and immobilize tyrosinase. Optimum conditions for the electropolymerization modified electrode were a temperature of 30 °C and a pH of 7 producing L-DOPA concentration 0.315 mM. After 40 days, the relative activity of an enzyme for electropolymerization remained 38.6%, respectively.

Original languageEnglish
Pages (from-to)817-821
Number of pages5
JournalKorean Chemical Engineering Research
Volume54
Issue number6
DOIs
Publication statusPublished - 1 Dec 2016

Keywords

  • Electrode surface
  • L-dopa synthesis
  • Parkinson's disease
  • Tyrosinase-immobilized

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