Kinetic study of Escherichia coli BPPTCC-EgRK2 to produce recombinant cellulase for ethanol production from oil palm empty fruit bunch

S. Limoes, S. F. Rahman, S. Setyahadi, Misri

Research output: Contribution to journalConference articlepeer-review

2 Citations (Scopus)

Abstract

Oil Palm Empty Fruit Bunch (OPEFB) is an abundant biomass resource in Indonesia, which contains 46,77% (w/w) of cellulose. The high cellulose content of OPEFB can be used as a substrate for bacteria cultivation to produce cellulase. By using OPEFB as an alternative substrate, the production cost of cellulase in industrial scale can be suppressed. However, currently there are no available research that simulate a cellulase production plant design. Prior to simulating the cellulase plant design, kinetic studies of bacteria used in cultivation are needed to create an accurate simulation. In this research, kinetic studies of E. coli BPPTCC-EgRK2 growth were examined with the Monod approach to get the Monod constant (Ks) and maximum specific growth rate (μmax). This study found that E. coli BPPTCC-EgRK2 have μmax and Ks of 1.581 and 0.0709 respectively. BPPTCC-EgRK2 produced intracellular cellulase, thus gave linear correlation between cell concentration and cellulase production.

Original languageEnglish
Article number012016
JournalIOP Conference Series: Earth and Environmental Science
Volume141
Issue number1
DOIs
Publication statusPublished - 6 Apr 2018
Event2nd International Conference on Biomass: Toward Sustainable Biomass Utilization for Industrial and Energy Applications, ICB 2017 - Bogor, Indonesia
Duration: 24 Jul 201725 Jul 2017

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