Kinetic study of cholesterol oxidation by cholesterol oxidase enzyme as application for cholesterol biosensor

Meka Saima Perdani, Muhamad Sahlan, Siti Farida, Dwini Normayulisa Putri, Sri Angky Soekanto, Heri Hermansyah

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

2 Citations (Scopus)

Abstract

Cholesterol oxidase enzyme well known as oxidoreductase enzyme which able to catalyze the transfer of electron from one molecule (the oxidant) to another molecule (the reductant). The kinetic model for cholesterol oxidation by cholesterol oxidase has been demonstrated using first order irreversible reaction with Euler's method. The aim of this study is to determine the kinetic parameter of cholesterol oxidation using enzyme cholesterol oxidase. For this kinetic study, experimental data was obtained from several literatures. The affecting factors of kinetic rate constant in this study were solvent addition, stabilizer concentration, granule concentration, temperature and immobilized enzyme. In the oxidation reaction, there were some variables condition based on the experimental data, CA-1, CA-2, CA-3, respectively. The modelling result showed that the first order reaction was able to modelling the cholesterol oxidation with high accuracy. The smallest relative error of kinetic value was showed in the immobilized enzyme with the temperature variable at 37°C with k value 0.0290h -1 . This kinetic study is useful for further developing of application for biosensor based enzyme as catalyzer in the oxidation reaction. Moreover, kinetic study is also useful for choosing the operating factor for cholesterol oxidation.

Original languageEnglish
Title of host publication3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices
Subtitle of host publicationProceedings of the International Symposium of Biomedical Engineering, ISBE 2018
EditorsPraswasti P.D.K. Wulan, Misri Gozan, Sotya Astutiningsih, Ghiska Ramahdita, Radon Dhelika, Prasetyanugraheni Kreshanti
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735418226
DOIs
Publication statusPublished - 9 Apr 2019
Event3rd International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2018 - Jakarta, Indonesia
Duration: 6 Aug 20188 Aug 2018

Publication series

NameAIP Conference Proceedings
Volume2092
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference3rd International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2018
Country/TerritoryIndonesia
CityJakarta
Period6/08/188/08/18

Keywords

  • biosensor
  • cholesterol
  • cholesterol oxidase
  • kinetic
  • modeling
  • oxidation

Fingerprint

Dive into the research topics of 'Kinetic study of cholesterol oxidation by cholesterol oxidase enzyme as application for cholesterol biosensor'. Together they form a unique fingerprint.

Cite this