TY - GEN
T1 - Kinetic study of cholesterol oxidation by cholesterol oxidase enzyme as application for cholesterol biosensor
AU - Perdani, Meka Saima
AU - Sahlan, Muhamad
AU - Farida, Siti
AU - Putri, Dwini Normayulisa
AU - Soekanto, Sri Angky
AU - Hermansyah, Heri
N1 - Publisher Copyright:
© 2019 Author(s).
PY - 2019/4/9
Y1 - 2019/4/9
N2 -
Cholesterol oxidase enzyme well known as oxidoreductase enzyme which able to catalyze the transfer of electron from one molecule (the oxidant) to another molecule (the reductant). The kinetic model for cholesterol oxidation by cholesterol oxidase has been demonstrated using first order irreversible reaction with Euler's method. The aim of this study is to determine the kinetic parameter of cholesterol oxidation using enzyme cholesterol oxidase. For this kinetic study, experimental data was obtained from several literatures. The affecting factors of kinetic rate constant in this study were solvent addition, stabilizer concentration, granule concentration, temperature and immobilized enzyme. In the oxidation reaction, there were some variables condition based on the experimental data, CA-1, CA-2, CA-3, respectively. The modelling result showed that the first order reaction was able to modelling the cholesterol oxidation with high accuracy. The smallest relative error of kinetic value was showed in the immobilized enzyme with the temperature variable at 37°C with k value 0.0290h
-1
. This kinetic study is useful for further developing of application for biosensor based enzyme as catalyzer in the oxidation reaction. Moreover, kinetic study is also useful for choosing the operating factor for cholesterol oxidation.
AB -
Cholesterol oxidase enzyme well known as oxidoreductase enzyme which able to catalyze the transfer of electron from one molecule (the oxidant) to another molecule (the reductant). The kinetic model for cholesterol oxidation by cholesterol oxidase has been demonstrated using first order irreversible reaction with Euler's method. The aim of this study is to determine the kinetic parameter of cholesterol oxidation using enzyme cholesterol oxidase. For this kinetic study, experimental data was obtained from several literatures. The affecting factors of kinetic rate constant in this study were solvent addition, stabilizer concentration, granule concentration, temperature and immobilized enzyme. In the oxidation reaction, there were some variables condition based on the experimental data, CA-1, CA-2, CA-3, respectively. The modelling result showed that the first order reaction was able to modelling the cholesterol oxidation with high accuracy. The smallest relative error of kinetic value was showed in the immobilized enzyme with the temperature variable at 37°C with k value 0.0290h
-1
. This kinetic study is useful for further developing of application for biosensor based enzyme as catalyzer in the oxidation reaction. Moreover, kinetic study is also useful for choosing the operating factor for cholesterol oxidation.
KW - biosensor
KW - cholesterol
KW - cholesterol oxidase
KW - kinetic
KW - modeling
KW - oxidation
UR - http://www.scopus.com/inward/record.url?scp=85064828190&partnerID=8YFLogxK
U2 - 10.1063/1.5096731
DO - 10.1063/1.5096731
M3 - Conference contribution
AN - SCOPUS:85064828190
T3 - AIP Conference Proceedings
BT - 3rd Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices
A2 - Wulan, Praswasti P.D.K.
A2 - Gozan, Misri
A2 - Astutiningsih, Sotya
A2 - Ramahdita, Ghiska
A2 - Dhelika, Radon
A2 - Kreshanti, Prasetyanugraheni
PB - American Institute of Physics Inc.
T2 - 3rd International Symposium of Biomedical Engineering''s Recent Progress in Biomaterials, Drugs Development, and Medical Devices, ISBE 2018
Y2 - 6 August 2018 through 8 August 2018
ER -