Kinetic studies of partially purified bromelain from Bogor pineapple (Ananas comosus [L.] Merr) core with ion exchange chromatography and in vitro evaluation of its antiplatelet activity

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Abstract

Bromelain is a mixture of the proteolitic enzymes found in pineapple plant tissues within the Bromeliaceous family. In this research the isolation and purification of bromelain from Bogor pineapple [Ananas comosus (L.) Merr] core were carried out using ammonium sulphate precipitation and Diethylaminoethy-Cellulose (DEAE-Cellulose) chromatography. The enzyme activities were evaluated using casein as substrate. The highest specific activity of bromelain from ammonium sulphate fractionation was obtained at 51.75 U/mg in the range of 20-80% saturation with a purity level of enzyme 24 times from its crude extract. Later on, the purification with DEAE-Cellulose resulted in the increasing of specific activity to 60.57 U/mg with a purity level of enzyme 28 times from its crude extract. Hydrolysis of various casein concentrations with purified bromelain was carried out at the optimum reaction condition of pH 7.0 and 37°C. The results obtained revealed the K m and Vmax values were 0.61% (w/v) and 6.22 U/min, respectively. In vitro study of antiplatelet agent activity using human Platelet Rich Plasma (PRP) revealed that all bromelain fractions showed the activity as an antiplatelet agent. The highest inhibition was shown by DEAE-Cellulose fraction of 54.16% with IC 50 of 31.37μL/mL.

Original languageEnglish
Title of host publicationProceedings of the 3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
EditorsRatna Yuniati, Terry Mart, Ivandini T. Anggraningrum, Djoko Triyono, Kiki A. Sugeng
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735417410
DOIs
Publication statusPublished - 22 Oct 2018
Event3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017 - Bali, Indonesia
Duration: 26 Jul 201727 Jul 2017

Publication series

NameAIP Conference Proceedings
Volume2023
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
Country/TerritoryIndonesia
CityBali
Period26/07/1727/07/17

Keywords

  • antiplatelet
  • bromelain
  • enzyme kinetics
  • pineapple core

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