Background Since its introduction in 1995, laparoscopic living donor nephrectomy (LLDN) has been a standard procedure in many transplantation centers worldwide. In 2011, LLDN was performed for the first time in Indonesia and became a standard procedure at our institution. The aim of this study is to evaluate the first 250 cases of LLDN at our institution. Methods Retrospective analyses were conducted of the first 250 cases of LLDN. All operations were performed between November 2011 and July 2015 at Cipto Mangunkusumo Hospital, Jakarta. We grouped each 100 consecutive cases and the last 50 cases (three groups) to evaluate operation-related parameters, and complications. Results Significant decreases in the first warm ischemic time, blood loss, length of stay, and number of complications were observed among the three groups. There was only one conversion to open case during LLDN surgery and no mortality case observed. Conclusion Laparoscopic living donor nephrectomies had been performed successfully with improvement of surgical parameter in due time. Thus, laparoscopic approach has entered a new era for kidney procurement in Indonesia.