Javanese turmeric (Curcuma xanthorrhixza Roxb.) ethanol extract has inhibitory effect on the development of intermediate phase of candida albicans biofilm

Javanese turmeric (Curcuma canthorrhiza Roxb.) is an Indonesian native medicinal plant that contains xanthorrhizol, which is known to have antifungal effect. After adhering to a surface in the early phase, the C. albicans biofilm develops into intermediate phase during which it grows filaments and transforms from yeast cells into hypha, so that it becomes more virulent. We aimed to analyze the potential effect of Javanese turmeric (Cucurma xanthorrhiza Roxb.) extract in inhibiting the intermediate phase of C. albicans biofilm. The Javanese turmeric ethanol extract was exposed to C. albicans ATCC 10231 biofilm aged 1.5 h and incubated in 37 °C for 24 h to achieve intermediate phase. MTT assay was used to assess the viability of C. albicans biofilm. The minimum biofilm inhibitory concentration (MBIC50) of Javanese turmeric ethanol extract against C. albicans ATCC 10231 in the intermediate phase was 35%. Javanese turmeric ethanol extract showed potential to inhibit C. albicans biofilm formation in the intermediate phase.