ITS regions of rDNA sequence and morphological analyses clarify five Rhizopus strains from tempeh as Rhizopus oryzae

R. Febriani, Wellyzar Sjamsuridzal, Ariyanti Oetari, Iman Santoso, I. G. Roosheroe

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

1 Citation (Scopus)

Abstract

The Rhizopus strains in the Universitas Indonesia Culture Collection (UICC) were isolated from tempeh from various regions in Indonesia. Five strains of Rhizopus (UICC 10, UICC 85, UICC 119, UICC 120, and UICC 135) were previously identified based on phenotypic characters (morphology and physiology) as R. oryzae. The aim of this study was to clarify the identities of five Rhizopus strains based on internal transcribed spacer (ITS) regions of ribosomal DNA sequence data. Molecular phylogeny was carried out to establish the identities of these five strains. Primer set ITS5 and ITS4 were used for amplification and sequencing of the ITS regions of rDNA. The sequence data was sent to genBank for homology search using basic local alignment search tool (BLAST) program. Construction of phylogenetic tree was conducted using Neighbor-Joining method with Kimura's two-parameter model. The results on gel electrophoresis showed that sizes of the ITS regions of rDNA fragment lengths of all strains were approximately 650 bp. The BLAST homology search results of five strains showed 99.8% homology to R. oryzae CBS 112.07T type strain. The phylogenetic tree clearly indicated that these five strains were included in a monophyletic group with R. oryzae CBS 112.07T type strain, and it was supported by high bootstrap value (84%). Morphological observation of all strains showed the characters corresponded to R. oryzae. The ITS regions of rDNA sequences and morphological characters have confirmed the strains as R. oryzae.

Original languageEnglish
Title of host publicationProceedings of the 3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
EditorsRatna Yuniati, Terry Mart, Ivandini T. Anggraningrum, Djoko Triyono, Kiki A. Sugeng
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735417410
DOIs
Publication statusPublished - 22 Oct 2018
Event3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017 - Bali, Indonesia
Duration: 26 Jul 201727 Jul 2017

Publication series

NameAIP Conference Proceedings
Volume2023
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference3rd International Symposium on Current Progress in Mathematics and Sciences 2017, ISCPMS 2017
Country/TerritoryIndonesia
CityBali
Period26/07/1727/07/17

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