Isolation, transformation, anticancer, and apoptosis activity of lupeyl acetate from Artocarpus integra

Hery Suwito, Wan Lelly Heffen, Antonius Herry Cahyana, Wahyudi Priyono Suwarso

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Lupeyl acetate-A major constituent of the bark of Artocarpus integra-was isolated and then transformed chemically into lupeol and lupenone by hydrolysis and oxidation reaction respectively. The molecular structures of the prepared compounds were determined based on FTIR, MS and NMR spectrum evidences. Their anticancer activities were determined against breast cancer cells MCF-7 using neutral red assay, while their apoptotic activity were confirmed by flowcytometric analysis using Annexin V-FTIC assay and DNA fragmentation. The IC50 of Lupeyl acetate, lupeol, and lupenone were 48.79; 43.09; and 8.07?μg/mL respectively. The results of flowcytometric analysis and DNA fragmentation showed that anticancer activity of the prepared compounds following apoptosis mechanism.

Original languageEnglish
Title of host publication5th International Conference and Workshop on Basic and Applied Sciences, ICOWOBAS 2015
EditorsMoh. Yasin, Sulaiman W. Harun
PublisherAmerican Institute of Physics Inc.
ISBN (Electronic)9780735413641
DOIs
Publication statusPublished - 15 Mar 2016
Event5th International Conference and Workshop on Basic and Applied Sciences, ICOWOBAS 2015 - Surabaya, Indonesia
Duration: 16 Oct 201517 Oct 2015

Publication series

NameAIP Conference Proceedings
Volume1718
ISSN (Print)0094-243X
ISSN (Electronic)1551-7616

Conference

Conference5th International Conference and Workshop on Basic and Applied Sciences, ICOWOBAS 2015
CountryIndonesia
CitySurabaya
Period16/10/1517/10/15

Keywords

  • anticancer
  • apoptosis.
  • lupenone
  • lupeol
  • lupeyl acetate

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