Isolation, purification, and characterization of porcine skin collagen: Analysis of the glycine, proline, and hydroxyproline components using high-performance liquid chromatography

Ayu Aditya Andayani, Harmita Harmita, Baitha Palanggatan Maggadani

Research output: Contribution to journalArticle

Abstract

Objective: The aim of this study is to produce collagen through the extraction and isolation of porcine skin. Methods: Collagen from porcine skin (Sus scrofa domesticus) was isolated, purified, and characterized. Major amino acid content of collagen (glycine, proline, and hydroxyproline) was determined. Samples were extracted with 0.5 N acetic acid and precipitated with 0.9 M NaCl. Characterization tests included those to determine the organoleptic content, pH, Fourier-transform infrared analysis, moisture content, ash content, viscosity, and Masson’s trichrome staining on collagen tissue. The collagen was further analyzed using high-performance liquid chromatography using C-18® column and a fluorescence detector at 265 nm and 320 nm, acetic buffer (pH 4.2)-acetonitrile (55:45) as mobile phase, and optimum flow rate of 0.8 mL/min. Results: Our findings indicated that the best method for isolating collagen was with 0.1 M NaOH expressed by average contents of glycine, proline, and hydroxyproline in collagen which were 33.663±0.215%, 12.333±0.128%, and 11.303±0.354%, respectively. Conclusion: Porcine collagen has been successfully obtained with this method.

Original languageEnglish
Pages (from-to)294-298
Number of pages5
JournalInternational Journal of Applied Pharmaceutics
Volume10
Issue numberSpecial Issue 1
DOIs
Publication statusPublished - 1 Dec 2018

Keywords

  • Amino acid
  • Derivatization
  • Fluorescence
  • Glycine
  • High-performance liquid chromatography
  • Hydroxyproline
  • Porcine skin collagen
  • Praline

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