TY - JOUR
T1 - Isolation of endophytic fungi from Brucea javanica L. (Merr.) and cytotoxic evaluation of their n-butanol extract from fermentation broth
AU - Kumala, Shirly
AU - Utji, Robert
AU - Sudarmono, Pratiwi Pudjilestari
AU - Kardono, Leonardus B.S.
PY - 2006/5
Y1 - 2006/5
N2 - Isolation of endophytic fungi from Brucea javanica L. (Merr) and leukemia cell L1210 cytotoxic evaluation of their n-butanol extract of the fermentation broth were carried out. Twigs, leaves and fruits of B. Javanica were collected randomly from three locations in Indonesia: Bogor, Cianjur and Tawangmangu. All specimens were cut and surface sterilized by using ethanol and sodium hypochlorite solution. Direct seed inoculating technique and Corn Meal Malt were used. For potential secondary metabolites evaluation was conducted by liquid fermentation using potato dextrose-yeast extract broth (PDY) and extracted in n-butanol. In this study, endophytic fungi were isolated; eleven isolates from Bogor, sixteen isolates from Cianjur and eighteen isolates from Tawangmangu. Cytotoxic assay were performed on n-butanol extract generated from the fermentation broth. The IC50 of the n-butanol extracts from 18 endophytic fungi isolates ranged from 3.29-15.90 ug mL-1. This results demonstrated that secondary metabolites of the n-butanol extract of those endophytic fungi may act as a potent anti cancer substance.
AB - Isolation of endophytic fungi from Brucea javanica L. (Merr) and leukemia cell L1210 cytotoxic evaluation of their n-butanol extract of the fermentation broth were carried out. Twigs, leaves and fruits of B. Javanica were collected randomly from three locations in Indonesia: Bogor, Cianjur and Tawangmangu. All specimens were cut and surface sterilized by using ethanol and sodium hypochlorite solution. Direct seed inoculating technique and Corn Meal Malt were used. For potential secondary metabolites evaluation was conducted by liquid fermentation using potato dextrose-yeast extract broth (PDY) and extracted in n-butanol. In this study, endophytic fungi were isolated; eleven isolates from Bogor, sixteen isolates from Cianjur and eighteen isolates from Tawangmangu. Cytotoxic assay were performed on n-butanol extract generated from the fermentation broth. The IC50 of the n-butanol extracts from 18 endophytic fungi isolates ranged from 3.29-15.90 ug mL-1. This results demonstrated that secondary metabolites of the n-butanol extract of those endophytic fungi may act as a potent anti cancer substance.
KW - Brucea javanica l. (merr)
KW - Cytotoxicity assay
KW - Endophytic fungi
KW - N-butanol extract
KW - Secondary metabolite
UR - http://www.scopus.com/inward/record.url?scp=33745323087&partnerID=8YFLogxK
U2 - 10.3923/pjbs.2006.825.832
DO - 10.3923/pjbs.2006.825.832
M3 - Article
AN - SCOPUS:33745323087
VL - 9
SP - 825
EP - 832
JO - Pakistan Journal of Biological Sciences
JF - Pakistan Journal of Biological Sciences
SN - 1028-8880
IS - 5
ER -