Isolation of endophytic fungi from Brucea javanica L. (Merr.) and cytotoxic evaluation of their n-butanol extract from fermentation broth

Isolation of endophytic fungi from Brucea javanica L. (Merr) and leukemia cell L1210 cytotoxic evaluation of their n-butanol extract of the fermentation broth were carried out. Twigs, leaves and fruits of B. Javanica were collected randomly from three locations in Indonesia: Bogor, Cianjur and Tawangmangu. All specimens were cut and surface sterilized by using ethanol and sodium hypochlorite solution. Direct seed inoculating technique and Corn Meal Malt were used. For potential secondary metabolites evaluation was conducted by liquid fermentation using potato dextrose-yeast extract broth (PDY) and extracted in n-butanol. In this study, endophytic fungi were isolated; eleven isolates from Bogor, sixteen isolates from Cianjur and eighteen isolates from Tawangmangu. Cytotoxic assay were performed on n-butanol extract generated from the fermentation broth. The IC₅₀ of the n-butanol extracts from 18 endophytic fungi isolates ranged from 3.29-15.90 ug mL^-1. This results demonstrated that secondary metabolites of the n-butanol extract of those endophytic fungi may act as a potent anti cancer substance.