Isolation of endophytic fungi from Brucea javanica L. (Merr.) and cytotoxic evaluation of their n-butanol extract from fermentation broth

Shirly Kumala, Robert Utji, Pratiwi Pudjilestari Sudarmono, Leonardus B.S. Kardono

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3 Citations (Scopus)

Abstract

Isolation of endophytic fungi from Brucea javanica L. (Merr) and leukemia cell L1210 cytotoxic evaluation of their n-butanol extract of the fermentation broth were carried out. Twigs, leaves and fruits of B. Javanica were collected randomly from three locations in Indonesia: Bogor, Cianjur and Tawangmangu. All specimens were cut and surface sterilized by using ethanol and sodium hypochlorite solution. Direct seed inoculating technique and Corn Meal Malt were used. For potential secondary metabolites evaluation was conducted by liquid fermentation using potato dextrose-yeast extract broth (PDY) and extracted in n-butanol. In this study, endophytic fungi were isolated; eleven isolates from Bogor, sixteen isolates from Cianjur and eighteen isolates from Tawangmangu. Cytotoxic assay were performed on n-butanol extract generated from the fermentation broth. The IC50 of the n-butanol extracts from 18 endophytic fungi isolates ranged from 3.29-15.90 ug mL-1. This results demonstrated that secondary metabolites of the n-butanol extract of those endophytic fungi may act as a potent anti cancer substance.

Original languageEnglish
Pages (from-to)825-832
Number of pages8
JournalPakistan Journal of Biological Sciences
Volume9
Issue number5
DOIs
Publication statusPublished - 1 May 2006

Keywords

  • Brucea javanica l. (merr)
  • Cytotoxicity assay
  • Endophytic fungi
  • N-butanol extract
  • Secondary metabolite

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