Isolation of cellulolytic fungi and utilization of its cellulolytic activity for microcrystalline cellulose preparation from water hyacinth (eichhornia crassipes)

Mitayani Wahyu Murti, Monica Angeline Sudarsono, Herman Suryadi

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Introduction: Microcrystalline cellulose is a cellulase derivative which usually used as a pharmaceutical excipient in the manufacturing of direct compression tablet. High concentration of cellulose is found in water hyacinth (about 60%) therefore it might be used in the manufacturing of microcrystalline cellulose. This study was aimed to obtain the best cellulolytic fungi, the best optimal conditions of enzymatic hydrolysis and comparing microcrystalline cellulose characteristics obtained from water hyacinth with microcrystalline cellulose standard, Avicel pH 101. Method: This study began with isolation of cellulolytic fungi, and then enzymatic hydrolysis of α-cellulose was done with cellulase enzymes extracted from cellulolytic fungi with various pH, temperature, duration and enzyme concentration. Microcrystalline obtained was characterized by XRD (X-Ray Diffraction) and then the result was compared with Avicel pH 101. Results: The results showed the best cellulolytic isolate was isolate 2 and its optimal hydrolysis conditions at pH 5, 300C, for 1 h with 5 mL (5% v/v) enzyme. Based on the pattern of diffraction there was a similarity between microcrystalline cellulose of enzymatic hydrolysis result compared with Avicel pH 101.

Original languageEnglish
Pages (from-to)1082-1088
Number of pages7
JournalPharmacognosy Journal
Volume10
Issue number6
DOIs
Publication statusPublished - 1 Nov 2018

Keywords

  • Cellulase
  • Cellulolytic fungi
  • Cellulose
  • Enzymatic hydrolysis
  • Microcrystalline cellulose
  • Water hyacinth

Fingerprint Dive into the research topics of 'Isolation of cellulolytic fungi and utilization of its cellulolytic activity for microcrystalline cellulose preparation from water hyacinth (eichhornia crassipes)'. Together they form a unique fingerprint.

  • Cite this