Isolation of cellular revertants from a rat cell line transformed by the E6 and E7 genes of human papillomavirus type 16

Tjahjani Mirawati Sudiro; Hirokazu Inoue; Masud Yutsudo; Akira Hakura

doi:10.1016/0042-6822(91)90681-Z

Isolation of cellular revertants from a rat cell line transformed by the E6 and E7 genes of human papillomavirus type 16

Tjahjani Mirawati Sudiro, Hirokazu Inoue, Masud Yutsudo, Akira Hakura

Department of Microbiology Clinic

Research output: Contribution to journal › Article › peer-review

2 Citations (Scopus)

Abstract

Three revenants defective in the ability to form colonies in semisolid medium were isolated from a rat cell line transformed by the E6 and E7 genes of human papillomavirus type 16 (HPV16). These revenants appeared to be defective in a cellular factor(s) necessary for transformation by HPV16-E6E7 genes since they still expressed a comparable amount of HPV16-E6E7 mRNA and E7 protein to the parental cells, harbored rescuable transforming virus, and were resistant to retransformation by HPV16-E6E7 genes. All these reverted phenotypes of the three mutants were recessive on somatic cell hybridization with normal cells, because all the hybrids showed transformed phenotypes.

Original language	English
Pages (from-to)	357-360
Number of pages	4
Journal	Virology
Volume	182
Issue number	1
DOIs	https://doi.org/10.1016/0042-6822(91)90681-Z
Publication status	Published - May 1991

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abstract = "Three revenants defective in the ability to form colonies in semisolid medium were isolated from a rat cell line transformed by the E6 and E7 genes of human papillomavirus type 16 (HPV16). These revenants appeared to be defective in a cellular factor(s) necessary for transformation by HPV16-E6E7 genes since they still expressed a comparable amount of HPV16-E6E7 mRNA and E7 protein to the parental cells, harbored rescuable transforming virus, and were resistant to retransformation by HPV16-E6E7 genes. All these reverted phenotypes of the three mutants were recessive on somatic cell hybridization with normal cells, because all the hybrids showed transformed phenotypes.",

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AU - Yutsudo, Masud

AU - Hakura, Akira

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AB - Three revenants defective in the ability to form colonies in semisolid medium were isolated from a rat cell line transformed by the E6 and E7 genes of human papillomavirus type 16 (HPV16). These revenants appeared to be defective in a cellular factor(s) necessary for transformation by HPV16-E6E7 genes since they still expressed a comparable amount of HPV16-E6E7 mRNA and E7 protein to the parental cells, harbored rescuable transforming virus, and were resistant to retransformation by HPV16-E6E7 genes. All these reverted phenotypes of the three mutants were recessive on somatic cell hybridization with normal cells, because all the hybrids showed transformed phenotypes.

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Isolation of cellular revertants from a rat cell line transformed by the E6 and E7 genes of human papillomavirus type 16

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