ISOLATION, IDENTIFICATION, AND ANTIBACTERIAL OF AMENTOFLAVONE FROM GARCINIA LATISSIMA MIQ. LEAVES

Neneng Siti Silfi Ambarwati; Berna Elya; Amarila Malik; Muhammad Hanafi; Muhamad Sharul Nizam Awang

doi:10.22159/ijap.2022.v14s3.13

ISOLATION, IDENTIFICATION, AND ANTIBACTERIAL OF AMENTOFLAVONE FROM GARCINIA LATISSIMA MIQ. LEAVES

Neneng Siti Silfi Ambarwati, Berna Elya, Amarila Malik, Muhammad Hanafi, Muhamad Sharul Nizam Awang

Department of Pharmacy

Research output: Contribution to journal › Article › peer-review

Abstract

Objective: This study aimed to isolate and identify the Bacillus subtilis antibacterial compound present in the leaves of the Garcinia latissima Miq. Methods: The extracts were obtained by maceration successively, and the active extract was fractionated by column chromatography. The isolation of the most active fraction was performed by open column chromatography and preparative thin-layer chromatography. The isolate antibacterial assay against Bacillus subtilis by microdilution method. The compound structure of the isolate was identified by the nuclear magnetic resonance method. Results: The most active extract of the extracts from Garcinia latissima Miq. leaves against Bacillus subtilis are the methanol extract. The most active fractions of the fractions from G. latissima Miq. Leaves methanolic extract was isolated. The active isolate was identified as Amentoflavone. Conclusion: G. latissima Miq. leaves had the potential to be used as antibacterial medicinal herbs and one of the isolates of G. latissima Miq. is amentoflavone (active against B. Subtilis).

Original language	English
Pages (from-to)	67-73
Number of pages	7
Journal	International Journal of Applied Pharmaceutics
Volume	14
Issue number	Special Issue 3
DOIs	https://doi.org/10.22159/ijap.2022.v14s3.13
Publication status	Published - Jun 2022

Keywords

Amentoflavone
Bacillus subtilis
Garcinia latissima Miq

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10.22159/ijap.2022.v14s3.13

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title = "ISOLATION, IDENTIFICATION, AND ANTIBACTERIAL OF AMENTOFLAVONE FROM GARCINIA LATISSIMA MIQ. LEAVES",

abstract = "Objective: This study aimed to isolate and identify the Bacillus subtilis antibacterial compound present in the leaves of the Garcinia latissima Miq. Methods: The extracts were obtained by maceration successively, and the active extract was fractionated by column chromatography. The isolation of the most active fraction was performed by open column chromatography and preparative thin-layer chromatography. The isolate antibacterial assay against Bacillus subtilis by microdilution method. The compound structure of the isolate was identified by the nuclear magnetic resonance method. Results: The most active extract of the extracts from Garcinia latissima Miq. leaves against Bacillus subtilis are the methanol extract. The most active fractions of the fractions from G. latissima Miq. Leaves methanolic extract was isolated. The active isolate was identified as Amentoflavone. Conclusion: G. latissima Miq. leaves had the potential to be used as antibacterial medicinal herbs and one of the isolates of G. latissima Miq. is amentoflavone (active against B. Subtilis).",

keywords = "Amentoflavone, Bacillus subtilis, Garcinia latissima Miq",

author = "Ambarwati, {Neneng Siti Silfi} and Berna Elya and Amarila Malik and Muhammad Hanafi and Awang, {Muhamad Sharul Nizam}",

note = "Funding Information: National Collaborative Research Grant 2021, Jakarta State University, Grant Number: 3/PKM/lPPM/IV/2021. AUTHORS CONTRIBUTIONS. Funding Information: National Collaborative Research Grant 2021, University, Grant Number: 3/PKM/lPPM/IV/2021. Funding Information: the National Collaborative University, Grant Number: Publisher Copyright: {\textcopyright} 2022 The Authors.",

year = "2022",

month = jun,

doi = "10.22159/ijap.2022.v14s3.13",

language = "English",

volume = "14",

pages = "67--73",

journal = "International Journal of Applied Pharmaceutics",

issn = "0975-7058",

publisher = "International Journal of Applied Pharmaceutics",

number = "Special Issue 3",

}

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T1 - ISOLATION, IDENTIFICATION, AND ANTIBACTERIAL OF AMENTOFLAVONE FROM GARCINIA LATISSIMA MIQ. LEAVES

AU - Ambarwati, Neneng Siti Silfi

AU - Elya, Berna

AU - Malik, Amarila

AU - Hanafi, Muhammad

AU - Awang, Muhamad Sharul Nizam

N1 - Funding Information: National Collaborative Research Grant 2021, Jakarta State University, Grant Number: 3/PKM/lPPM/IV/2021. AUTHORS CONTRIBUTIONS. Funding Information: National Collaborative Research Grant 2021, University, Grant Number: 3/PKM/lPPM/IV/2021. Funding Information: the National Collaborative University, Grant Number: Publisher Copyright: © 2022 The Authors.

PY - 2022/6

Y1 - 2022/6

N2 - Objective: This study aimed to isolate and identify the Bacillus subtilis antibacterial compound present in the leaves of the Garcinia latissima Miq. Methods: The extracts were obtained by maceration successively, and the active extract was fractionated by column chromatography. The isolation of the most active fraction was performed by open column chromatography and preparative thin-layer chromatography. The isolate antibacterial assay against Bacillus subtilis by microdilution method. The compound structure of the isolate was identified by the nuclear magnetic resonance method. Results: The most active extract of the extracts from Garcinia latissima Miq. leaves against Bacillus subtilis are the methanol extract. The most active fractions of the fractions from G. latissima Miq. Leaves methanolic extract was isolated. The active isolate was identified as Amentoflavone. Conclusion: G. latissima Miq. leaves had the potential to be used as antibacterial medicinal herbs and one of the isolates of G. latissima Miq. is amentoflavone (active against B. Subtilis).

AB - Objective: This study aimed to isolate and identify the Bacillus subtilis antibacterial compound present in the leaves of the Garcinia latissima Miq. Methods: The extracts were obtained by maceration successively, and the active extract was fractionated by column chromatography. The isolation of the most active fraction was performed by open column chromatography and preparative thin-layer chromatography. The isolate antibacterial assay against Bacillus subtilis by microdilution method. The compound structure of the isolate was identified by the nuclear magnetic resonance method. Results: The most active extract of the extracts from Garcinia latissima Miq. leaves against Bacillus subtilis are the methanol extract. The most active fractions of the fractions from G. latissima Miq. Leaves methanolic extract was isolated. The active isolate was identified as Amentoflavone. Conclusion: G. latissima Miq. leaves had the potential to be used as antibacterial medicinal herbs and one of the isolates of G. latissima Miq. is amentoflavone (active against B. Subtilis).

KW - Amentoflavone

KW - Bacillus subtilis

KW - Garcinia latissima Miq

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ISOLATION, IDENTIFICATION, AND ANTIBACTERIAL OF AMENTOFLAVONE FROM GARCINIA LATISSIMA MIQ. LEAVES

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Keywords

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