Isolation and structure elucidation of Eumitrin A1 from lichen Usnea blepharea motyka and its cytotoxic activity

Maulidiyah, A. Herry Cahyana, Wahyudi Priyono Suwarso, Muhammad Nurdin

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The purpose of this research was to isolate and elucidate structure of secondary metabolite compoundand to examine its cytotoxic activity of lichen Usnea blepharea Motyka obtained from Sulawesi – Indonesia. Powder of lichen was extracted using an acetone and continued separation by employing column chromatography (CC) with n-hexane and ethyl acetate solvents in a gradient elutionto produce a pure isolate. The structure was determined by UV-Vis, LC-MS, FTIR, 1H-NMR, 13C-NMR, 2D-NMR (HMBC, HMQC, COSY). The UV-Visspectrophotometer measurement was obtained maximumwavelength of 229 nm and 334 nm. The LC-MS data of this compound showed a molecular weight of680. The 1H-NMR, 13C-NMR with DEPT spectrums showedthat this compound has 34 carbon atoms consisting of 2 methyl groups, 2 methoxy groups, 1 methyl group, 6 metin groups, 3 methylene groups, and 20 C quartener atoms. The2D-NMR (HMBC, HMQC,COSY) spectrums of analysis could be inferred that this compound is a Eumitrin A1 with the formula structure C34H32O15. The Eum itrin A1 was examined its cytotoxic activityagainst Murine Leukemia P388 cells with IC504.5 μg/mL (very active).

Original languageEnglish
Pages (from-to)782-789
Number of pages8
JournalInternational Journal of PharmTech Research
Issue number4
Publication statusPublished - 1 Jan 2015


  • Cytotoxic
  • Eumitrin A<inf>1</inf>
  • Murine Leukemia P388 Cells
  • Usnea blepharea Motyka


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