TY - GEN
T1 - Isolation and screening of extracellular enzymes producing bacteria from black soldier fly larvae
AU - Sugiyanto, Ahmad
AU - Tamrela, Helmy
AU - Santoso, Iman
AU - Fadhilah, Qonita Gina
N1 - Publisher Copyright:
© 2023 Author(s).
PY - 2023/6/2
Y1 - 2023/6/2
N2 - Hermetia illucens L. is known as Black Soldier Fly (BSF), a voracious feeders during larval stages. Its role as bioconversion of organic waste is assumed correlate with the bacteria in BSF larvae. The bacteria are expected to have enzyme activity such as amylase, lipase, and protease. In the present study, the bacteria from BSF larvae were isolated using 6 different media. The isolated bacteria will be screened for their amylolytic, lipolytic, and proteolytic activities. Based on the formation of a clear zone and good growth appearance, total 22 representative bacterial isolates were obtained. Characterization of the isolates was consist of the morphological and biochemical tests. According to the result of the enzymatic test, five isolates have positive result on the amylolytic test, two isolates have a positive result on the lipolytic test, and eleven isolates have a positive result on the proteolytic test. Among those isolates, the B18 isolate has positive result on the three enzymatic tests. Molecular identification based on 16S rRNA showed that B18 was closely related to Bacillus paramycoides with 99.93% sequences similarity.
AB - Hermetia illucens L. is known as Black Soldier Fly (BSF), a voracious feeders during larval stages. Its role as bioconversion of organic waste is assumed correlate with the bacteria in BSF larvae. The bacteria are expected to have enzyme activity such as amylase, lipase, and protease. In the present study, the bacteria from BSF larvae were isolated using 6 different media. The isolated bacteria will be screened for their amylolytic, lipolytic, and proteolytic activities. Based on the formation of a clear zone and good growth appearance, total 22 representative bacterial isolates were obtained. Characterization of the isolates was consist of the morphological and biochemical tests. According to the result of the enzymatic test, five isolates have positive result on the amylolytic test, two isolates have a positive result on the lipolytic test, and eleven isolates have a positive result on the proteolytic test. Among those isolates, the B18 isolate has positive result on the three enzymatic tests. Molecular identification based on 16S rRNA showed that B18 was closely related to Bacillus paramycoides with 99.93% sequences similarity.
UR - http://www.scopus.com/inward/record.url?scp=85163353514&partnerID=8YFLogxK
U2 - 10.1063/5.0140446
DO - 10.1063/5.0140446
M3 - Conference contribution
AN - SCOPUS:85163353514
T3 - AIP Conference Proceedings
BT - 11th International Seminar on New Paradigm and Innovation on Natural Sciences and Its Application, ISNPINSA 2021
A2 - Soesanto, Qidir Maulana Binu
A2 - Bima, Damar Nurwahyu
A2 - Prasetya, Nor Basid Adiwibawa
A2 - Sugito, Heri
A2 - Maulidiyah, Alik
PB - American Institute of Physics Inc.
T2 - 11th International Seminar on New Paradigm and Innovation on Natural Sciences and Its Application: Developing Science and Technology Toward the New Normal Era, ISNPINSA 2021
Y2 - 14 October 2021 through 15 October 2021
ER -